Cytotechnology. 1997 Nov;25(1-3):25-33. doi: 10.1023/A:1007935026770.
COS, myeloma and HeLa cells, which are commonly used for protein production by cell culture, were transfected with human bcl-2 gene encoded on the shuttle vector BCMGS. Expression of human bcl-2 improved survival of cells remarkably, mildly, or negligibly for COS, myeloma, and HeLa, respectively. Four clones were obtained from the human bcl-2 expressing cell population of COS cells. They expressed human bcl-2 almost at the same level. The viable cell numbers were 6, 2.5, 2.5, and 0.8 times as many for the clones #8, #5, #6, and #7, respectively, as for the control COS cells, when they were cultured at low (0.2%) serum concentration for 9 days. The bcl-2 overexpressing COS cells showed morphology different from that of the control COS cells in serum limited condition. When transfected with mouse lambda protein gene carried by an SV40-derived vector, clone #8 of the bcl-2 transfected COS cells continued the transient expression of lambda protein longer than the control COS cells.
COS、骨髓瘤和 HeLa 细胞通常用于细胞培养生产蛋白质，这些细胞被转染了穿梭载体 BCMGS 上编码的人 bcl-2 基因。人 bcl-2 的表达显著、轻度或几乎不改善了 COS、骨髓瘤和 HeLa 细胞的存活率。从 COS 细胞的表达人 bcl-2 的细胞群体中获得了 4 个克隆。它们的人 bcl-2 表达水平几乎相同。当在低血清浓度（0.2%）下培养 9 天时，克隆 #8、#5、#6 和 #7 的活细胞数分别是对照 COS 细胞的 6、2.5、2.5 和 0.8 倍。在血清限制条件下，过表达 bcl-2 的 COS 细胞表现出与对照 COS 细胞不同的形态。当用 SV40 衍生载体携带的小鼠 lambda 蛋白基因转染时，bcl-2 转染的 COS 细胞克隆 #8 比对照 COS 细胞持续表达 lambda 蛋白的时间更长。
