刀豆球蛋白 A 固定化载体珠上肿瘤特异性细胞毒性 T 淋巴细胞的生长刺激作用。
Growth stimulation of tumor-specific cytotoxic T lymphocytes on concanavalin a-immobilized carrier beads.
机构信息
RIKEN Cell Bank, The Institute of Physical and Chemical Research (RIKEN), Koyadai 3-1-1, Tsukuba Science City, 305, Japan.
出版信息
Cytotechnology. 1998 Jan;26(1):13-21. doi: 10.1023/A:1007924430570.
Human tumor-specific CD4(+) cytotoxic T lymphocytes (CTL) were generated against duodenum papilloma cell line TGBC18TKB from HLA type-matched peripheral blood mononuclear cells. Concanavalin A (Con A) immobilized on carrier beads stimulated growth of the CTL in a long-term culture without repeated antigen stimulation, while soluble Con A induced death of the CTL. The CTL exhibited the target-specific cytotoxicity in a more potent manner than those before the long-term culture in the presence of the immobilized Con A. Enhanced expression of the adhesion molecule, CD11b, was observed on the CTL. These results suggest that immobilized Con A will be useful for continuous growth stimulation and large scale expansion of CTL without tumor antigen.
从与 HLA 匹配的外周血单核细胞中，针对十二指肠乳头瘤细胞系 TGBC18TKB，生成了人类肿瘤特异性 CD4(+)细胞毒性 T 淋巴细胞 (CTL)。刀豆蛋白 A (Con A)固定在载体珠上，在没有重复抗原刺激的长期培养中刺激 CTL 的生长，而可溶性 Con A 诱导 CTL 死亡。与长期培养前相比，在固定化 Con A 的存在下，CTL 以更有效的方式表现出针对靶标的细胞毒性。在 CTL 上观察到黏附分子 CD11b 的表达增强。这些结果表明，固定化 Con A 将有助于在没有肿瘤抗原的情况下，持续刺激 CTL 的生长并进行大规模扩增。
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