微载体培养：一种不同的颗粒细胞培养方法。

Microcarrier culture: A different approach to granulosa cell cultivation.

机构信息

Laboratory of Animal Endocrinology and Tissue Culture, Dept of Animal Physiology, Institute of Zoology, The Jagiellonian University, Ingardena 6, 30-060, Kraków, Poland.

出版信息

Cytotechnology. 1995 Jun;19(2):167-72. doi: 10.1007/BF00749772.

DOI:10.1007/BF00749772

PMID:22359016

Abstract

Granulosa cells (GC) are steroid secreting and hormone responsive cells synthesizing increased amounts of progesterone (P(4)) under the influence of gonadotropins. Up to now these cells have been cultivated as monolayers. However, in this system, cells dedifferentiate early and cease to respond to stimulation. GC are a good model for studying hormonal regulation of ovarian cell function. In this study, microcarrier culture was tested for the first time to see whether it would be a good system in which GC would grow and give a stronger responsivity to gonadotropins. Cells were grown in stationary culture on microcarriers Cytodex 3 (C3) coated with collagen and on pure gelatin beads. Only C3 cultures were successful. Cells proliferated better on C3 microcarriers and on day 4 of culture secreted more P(4) under the influence of FSH and LH than comparative monolayer cultures.

摘要

颗粒细胞（GC）是类固醇分泌细胞和激素反应细胞，在促性腺激素的影响下合成大量孕激素（P(4)）。到目前为止，这些细胞一直被作为单层细胞进行培养。然而，在这个系统中，细胞早期去分化并停止对刺激的反应。GC 是研究激素对卵巢细胞功能调节的良好模型。在这项研究中，首次测试了微载体培养，以观察其是否是一种良好的系统，使 GC 能够生长并对促性腺激素产生更强的反应性。细胞在涂有胶原蛋白的 Cytodex 3（C3）微载体上进行静止培养，并在纯明胶珠上进行培养。只有 C3 培养是成功的。细胞在 C3 微载体上更好地增殖，并且在培养的第 4 天，在 FSH 和 LH 的影响下分泌的 P(4)比对照的单层培养物多。