用流式细胞术分析昆虫细胞系的 DNA 含量。
DNA content analysis of insect cell lines by flow cytometry.
机构信息
Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Trois-Rivières (, Qc, G9A 5H7, Canada.
出版信息
Cytotechnology. 1999 Mar;29(2):103-13. doi: 10.1023/A:1008092620572.
Abstract
The DNA content of insect cell lines (6 lepidoptera, 1 coleoptera and 1 diptera) was determined by flow cytometry. The DNA profiles of the 8 cell lines tested were different. They were characterized by the presence of several peaks (2 to 7) corresponding to different ploidy levels, by differences in the fluorescence intensity of each peak and by the proportion of cells in each peak. Two cell lines (Cf124 and BmN) were constituted of 2 distinct populations of cells. The DNA profiles of the cell lines were stable among the passages and during the length of time culture. This technique was demonstrated to be useful for the detection of mixed cell lines and nucleopolyhedrovirus cell infection, using Autographa californica MNPV. The flow cytometry gives interesting results on the cell cycle and the ploidy level; it appears as a good tool for insect cell lines characterization.
摘要
通过流式细胞术测定了 8 种昆虫细胞系（6 种鳞翅目、1 种鞘翅目和 1 种双翅目）的 DNA 含量。所测试的 8 种细胞系的 DNA 图谱不同。它们的特征是存在几个峰（2 到 7），对应不同的倍性水平，每个峰的荧光强度不同，以及每个峰中细胞的比例不同。有 2 种细胞系（Cf124 和 BmN）由 2 个不同的细胞群体组成。细胞系的 DNA 图谱在传代和培养时间内保持稳定。该技术已被证明可用于检测混合细胞系和核多角体病毒细胞感染，使用的是美洲棉铃象鼻虫 MNPV。流式细胞术在细胞周期和倍性水平上给出了有趣的结果；它似乎是昆虫细胞系特征描述的一种很好的工具。
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