Agricultural Research Service, Insect Biocontrol Laboratory, U.S. Department of Agriculture, BARC-West, Bld 011A, Rm 214, 20705-2350, Beltsville, MD, U.S.A..
Cytotechnology. 1996 Jan;20(1-3):3-11. doi: 10.1007/BF00350384.
With the wide availability of insect cell culture media, it can generally be considered a routine process to develop new cell lines. Exceptions to this statement do exist, of course. Difficulties may arise when attempting to culture a specific cell type. For example, while there are a few cell lines from insect fat body and at least one from the midgut, it may not be possible to obtain cell lines from these tissues from all insect species due to terminal differentiation and other factors. Also, researchers have desired cell lines from certain species, such as the honey bee, for which no success has been obtained. As in the early days of tissue culture, it is difficult to discern why negative results occur. However, as more is learned about the physiology and nutrition of various insects and tissues, we may get clues which will help solve these questions.The remaining chapters in this book will provide the reader with exciting uses for insect cell culture. As I mentioned earlier, the baculovirus expression vector system has provided a stimulus to the field of insect cell culture not seen previously.
随着昆虫细胞培养基的广泛应用，开发新的细胞系通常可以被认为是一个常规过程。当然，这种说法也有例外。在尝试培养特定的细胞类型时，可能会遇到困难。例如，虽然有一些来自昆虫脂肪体的细胞系，至少有一种来自中肠，但由于终末分化和其他因素，并非所有昆虫物种都能从这些组织中获得细胞系。此外，研究人员希望获得某些物种的细胞系，例如蜜蜂，但迄今为止尚未成功。就像组织培养的早期一样，很难确定为什么会出现负面结果。然而，随着对各种昆虫和组织的生理学和营养的了解越来越多，我们可能会得到一些线索，帮助解决这些问题。本书的其余章节将为读者提供昆虫细胞培养的令人兴奋的用途。正如我之前提到的，杆状病毒表达载体系统为昆虫细胞培养领域带来了前所未有的刺激。
