Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France.
J Am Soc Mass Spectrom. 2012 May;23(5):869-79. doi: 10.1007/s13361-012-0341-y. Epub 2012 Feb 23.
Lipidomic studies often use liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) for separation, identification, and quantification. However, due to the wide structural diversity of lipids, the most apolar part of the lipidome is often detected with low sensitivity in ESI. Atmospheric pressure (APPI) can be an alternative ionization source since normal-phase solvents are known to enhance photoionization of these classes. In this paper, we intend to show the efficiency of APPI to identify different lipid classes, with a special interest on sphingolipids. In-source APPI fragmentation appears to be an added value for the structural analysis of lipids. It provides a detailed characterization of both the polar head and the non polar moiety of most lipid classes, and it makes possible the detection of all lipids in both polarities, which is not always possible with ESI.
脂质组学研究通常使用液相色谱/电喷雾电离质谱（LC/ESI-MS）进行分离、鉴定和定量。然而，由于脂质结构多样性广泛，脂质组中最非极性的部分在 ESI 中通常检测灵敏度较低。大气压（APPI）可以作为替代的离子源，因为众所周知，正相溶剂可以增强这些类别的光离子化。在本文中，我们旨在展示 APPI 鉴定不同脂质类别的效率，特别关注鞘脂。源内 APPI 碎裂似乎是脂质结构分析的一个附加价值。它提供了对大多数脂质类别的极性头和非极性部分的详细特征描述，并且可以检测到所有在两种极性下的脂质，这在 ESI 中并不总是可行的。
