Department of Proteomics, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany.
FEMS Microbiol Ecol. 2012 Jul;81(1):134-44. doi: 10.1111/j.1574-6941.2012.01334.x. Epub 2012 Mar 12.
A sulfate-reducing consortium maintained for several years in the laboratory with m-xylene as sole source of carbon and energy was characterized by terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of PCR-amplified 16S rRNA genes and stable isotope probing of proteins (Protein-SIP). During growth upon m-xylene or methyl-labeled m-xylene (1,3-dimethyl-(13)C(2)-benzene), a phylotype affiliated to the family Desulfobacteriaceae became most abundant. A second dominant phylotype was affiliated to the phylum Epsilonproteobacteria. In cultures grown with methyl-labeled m-xylene, 331 proteins were identified by LC-MS/MS analysis. These proteins were either not (13)C-labeled (23%) or showed a (13)C-incorporation of 19-22 atom% (13)C (77%), the latter demonstrating that methyl groups of m-xylene were assimilated. (13)C-labeled proteins were involved in anaerobic m-xylene biodegradation, in sulfate reduction, in the Wood-Ljungdahl-pathway, and in general housekeeping functions. Thirty-eight percent of the labeled proteins were affiliated to Deltaproteobacteria. Probably due to a lack of sequence data from Epsilonproteobacteria, only 14 proteins were assigned to this phylum. Our data suggest that m-xylene is assimilated by the Desulfobacteriaceae phylotype, whereas the role of the Epsilonproteobacterium in the consortium remained unclear.
硫酸盐还原菌群落经过数年的实验室培养，以间二甲苯作为唯一的碳源和能源，通过聚合酶链反应扩增 16S rRNA 基因的末端限制性片段长度多态性（T-RFLP）指纹图谱和蛋白质稳定同位素探测（Protein-SIP）进行了表征。在间二甲苯或甲基标记的间二甲苯（1,3-二甲基-(13)C(2)-苯）生长过程中，与脱硫杆菌科相关的一个生物型变得最为丰富。第二个主要生物型与ε变形菌门有关。在使用甲基标记的间二甲苯培养的培养物中，通过 LC-MS/MS 分析鉴定了 331 种蛋白质。这些蛋白质要么未被（13)C 标记（23%），要么显示（13)C 掺入率为 19-22 原子%（13)C（77%），后者表明间二甲苯的甲基被同化。（13)C 标记的蛋白质参与厌氧间二甲苯生物降解、硫酸盐还原、Wood-Ljungdahl 途径以及一般的维持功能。38%的标记蛋白与δ变形菌门有关。可能由于缺乏来自 ε变形菌门的序列数据，只有 14 种蛋白质被分配到该门。我们的数据表明，间二甲苯被脱硫杆菌生物型同化，而该群落中 ε变形菌的作用仍不清楚。
