Brigham and Women's Hospital, Harvard Medical School, 75 Francis St, Boston, MA 02115, USA.
Acta Obstet Gynecol Scand. 2012 May;91(5):613-9. doi: 10.1111/j.1600-0412.2012.01381.x. Epub 2012 Apr 5.
To compare cellular composition (fibroblasts vs. smooth muscle cells) and proliferation in uterine healing wounds after application of barbed compared with standard suture in a sheep model.
Randomized trial (Canadian Task Force classification I) using each animal as its own control.
Certified animal research facility. Population or sample. 23 non-pregnant ewes.
A myometrial incision was created with the harmonic scalpel in each horn of the bicornuate uterus. The incisions were randomly allocated to be closed using either polyglactin 210 (Vicryl®) or barbed suture. Three months later, uterine tissues were collected, fixed and used for determination of cellular composition and proliferation using histochemistry (Masson trichrome staining) and immunohistochemistry (staining of smooth muscle cell actin and Ki67, a marker of proliferating cells) followed by image analysis.
Evaluation and comparison of the cellular composition and proliferation of uterine wounds after application of barbed vs. standard suture.
The ratio between connective tissue elements and smooth muscle cells, expression of smooth muscle cell actin and labeling index were similar in wounds after application of barbed compared with standard suture, but were different (p < 0.0001-0.05) in wounds than in non-wounded areas in uterus.
Both barbed and standard sutures had similar effects on cellular composition and proliferation of uterine wounds in an animal model.
比较在绵羊模型中应用带倒刺缝线与标准缝线后子宫愈合伤口的细胞组成（成纤维细胞与平滑肌细胞）和增殖情况。
随机试验（加拿大任务组分类 I），每个动物作为自身对照。
经认证的动物研究设施。
23 只非妊娠母羊。
使用超声刀在双角子宫的每个角部创建子宫肌层切口。将切口随机分配使用聚甘醇酸 210（薇乔®）缝线或带倒刺缝线缝合。3 个月后，收集子宫组织，固定并用于通过组织化学（Masson 三色染色）和免疫组织化学（平滑肌细胞肌动蛋白和 Ki67 染色，增殖细胞的标志物）检测来确定细胞组成和增殖情况，随后进行图像分析。
比较带倒刺缝线与标准缝线应用后子宫伤口的细胞组成和增殖情况。
与标准缝线相比，带倒刺缝线应用后，结缔组织成分与平滑肌细胞的比例、平滑肌细胞肌动蛋白的表达和标记指数相似，但在子宫伤口处与非伤口处之间存在差异（p<0.0001-0.05）。
在动物模型中，带倒刺缝线和标准缝线对子宫伤口的细胞组成和增殖均具有相似的作用。
