Emergency Department and Surgical Intensive Care Unit, Shanghai 10th People's Hospital, Tongji University School of Medicine, China.
Burns. 2012 Aug;38(5):743-50. doi: 10.1016/j.burns.2011.12.026. Epub 2012 Feb 22.
Organ protection is a routine therapy in severe burn/scald injuries, and damage mechanisms following early scald injury was not been fully elucidated. Our aim was to verify the beneficial effects of Ligustrazine on pulmonary damage associated with scald injury. Lewis rats were subjected to 30% total body surface area (TBSA) scald injury, and were randomly divided into a burn control (S group) and an Ligustrazine-treated group (L group). Lung malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined and the lungs were examined histologically with immunohistochemistry (IHC) as well for the MHC class I chain-related antigen A (MICA) and Bcl-2 at 24, 48 and 72h after the injury. The expression of spleen HLA-DR was detected by immunohistochemistry analysis. Selectins and adhesion molecules in lungs and serum as well as pulmonary interleukins were also detected. The lung injury degree was represented as wet/dry (W/D) values and alveolar thickness. Ligustrazine decreased MDA levels and ameliorated the down-regulation of SOD activity. MICA was up-regulated after the scald, and this up-regulation was greatly diminished by Ligustrazine. Bcl-2 was up-regulated after the scald, especially in the L group. The spleen HLA-DR expression demonstrated the immunoregulatory effects of Ligustrazine, which effectively protected pulmonary tissues from scald-induced injury. Our results demonstrated that pulmonay damage associated with autoimmunity and oxidant attack occurred after severe scald. Ligustrazine exhibits significant protective effects on these effects.
器官保护是严重烧伤/烫伤患者的常规治疗方法，但早期烫伤损伤的机制尚未完全阐明。我们的目的是验证川芎嗪对烫伤相关肺损伤的有益作用。Lewis 大鼠接受 30%总体表面积（TBSA）烫伤损伤，随机分为烧伤对照组（S 组）和川芎嗪治疗组（L 组）。伤后 24、48 和 72 小时，测定肺组织丙二醛（MDA）和超氧化物歧化酶（SOD）水平，并通过免疫组织化学（IHC）检测肺组织中 MHC Ⅰ类链相关抗原 A（MICA）和 Bcl-2 的表达。通过免疫组化分析检测脾脏 HLA-DR 的表达。还检测了肺和血清中的选择素和粘附分子以及肺内白细胞介素。肺损伤程度用湿/干（W/D）值和肺泡厚度表示。川芎嗪降低 MDA 水平并改善 SOD 活性的下调。烫伤后 MICA 上调，川芎嗪可明显减少上调。烫伤后 Bcl-2 上调，尤其是在 L 组。脾脏 HLA-DR 的表达表明川芎嗪具有免疫调节作用，可有效保护肺组织免受烫伤损伤。我们的结果表明，严重烫伤后会发生与自身免疫和氧化剂攻击相关的肺损伤。川芎嗪对这些损伤具有显著的保护作用。
