Department of Chemical Engineering, McGill University, Montreal, QC, Canada.
Biotechnol Lett. 2012 Jun;34(6):1159-65. doi: 10.1007/s10529-012-0885-8. Epub 2012 Feb 24.
An electrochemistry-based method for the formation of functionalized alkanethiol layers on a 316L stainless steel surface was developed. The method was efficient in forming a very stable, irreversibly-attached COOH-terminated (mercaptoundecanoic acid) surface layer. This layer was used as a 'linker' to immobilize the extracellular matrix protein fibronectin to the 316L stainless steel surface. Fibronectin was irreversibly attached to the surface and, unlike physisorbed fibronectin, resisted detachment more in aggressive 0.1 M NaOH under sonication. The fibronectin-modified 316L stainless steel surface was more biocompatible towards attachment of endothelial cells than a bare (unmodified) 316L stainless steel surface, yielding a 25% improvement in cell density.
一种在 316L 不锈钢表面形成功能化烷硫醇层的基于电化学的方法被开发出来。该方法能够高效地形成非常稳定、不可逆附着的 COOH 端（巯基十一酸）表面层。该层可用作“链接器”将细胞外基质蛋白纤维连接蛋白固定到 316L 不锈钢表面。纤维连接蛋白不可逆地附着在表面上，与物理吸附的纤维连接蛋白不同，在超声作用下更能抵抗更具侵蚀性的 0.1 M NaOH 中的脱落。与裸（未修饰）316L 不锈钢表面相比，纤维连接蛋白修饰的 316L 不锈钢表面更有利于内皮细胞的附着，细胞密度提高了 25%。
