OCT4 在人食管鳞癌中的表达与预后不良显著相关。
Expression of OCT4 in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis.
机构信息
Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.
出版信息
World J Gastroenterol. 2012 Feb 21;18(7):712-9. doi: 10.3748/wjg.v18.i7.712.
AIM
To explore the expression pattern of OCT4 in human esophageal squamous cell carcinoma and its significance in diagnosis and prognosis.
METHODS
Using real-time polymerase chain reaction (PCR), Western blotting, immunocytochemistry and immunohistochemistry, the expression of OCT4 in three esophageal squamous cancer cell lines, KYSE70, KYSE140 and KYSE450, was characterized. OCT4 expression was investigated in a series of 153 esophageal squamous cell carcinoma samples using immunohistochemistry and explored its association with clinicopathological features.
RESULTS
Immunohistochemically, OCT4 positive immunostaining was observed in cancer cell nuclei. OCT4 was variably expressed in three esophageal squamous cancer cell lines. Among 153 specimens, 105 (68.7%) were negative or weakly positive for OCT4 staining; 21 (13.7%) were moderately positive and 27 (17.6%) were strongly positive. Higher expression level of OCT4 was significantly associated with higher histological grade (P < 0.001) and poor clinic outcome (P < 0.001).
CONCLUSION
The expression of OCT4 enables the tumor to have a higher degree of stemness, which in turn results in a poorer clinical outcome for patients with esophageal squamous cell carcinoma.
目的
探讨 OCT4 在人食管鳞癌中的表达模式及其在诊断和预后中的意义。
方法
采用实时聚合酶链反应（PCR）、Western blot、免疫细胞化学和免疫组织化学技术，对 3 种食管鳞癌细胞系 KYSE70、KYSE140 和 KYSE450 中 OCT4 的表达进行了鉴定。采用免疫组织化学方法检测了 153 例食管鳞癌标本中 OCT4 的表达，并探讨了其与临床病理特征的关系。
结果
免疫组化染色显示 OCT4 在癌细胞核中呈阳性表达。OCT4 在 3 种食管鳞癌细胞系中呈不同程度的表达。在 153 例标本中，105 例（68.7%） OCT4 染色阴性或弱阳性；21 例（13.7%）中度阳性，27 例（17.6%）强阳性。OCT4 表达水平较高与组织学分级较高（P < 0.001）和临床预后不良（P < 0.001）显著相关。
结论
OCT4 的表达使肿瘤具有更高的干性，进而导致食管鳞癌患者的临床预后较差。
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