Romani Sara, Azimzadeh Pedram, Mohebbi Seyed Reza, Kazemian Shabnam, Almasi Shohreh, Naghoosi Hamed, Derakhshan Faramarz, Zali Mohammad Reza
Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
Hepat Mon. 2011 Nov;11(11):901-6. doi: 10.5812/kowsar.1735143X.776. Epub 2011 Nov 20.
Chronic hepatitis C infection is caused by the hepatitis C virus (HCV), and its clinical complications include liver cirrhosis, liver failure, and hepatocellular carcinoma.Transforming growth factor-β1 (TGF-β1) is an important cytokine in cell growthand differentiation, angiogenesis, extracellular matrix formation, immune responseregulation, and cancer development and progression.
The aim of this study was to investigate the relationship between single nucleotide polymorphisms (SNPs) in TGF-β1 and chronic HCV infection among patients referred to the Taleghani Hospital, Tehran, Iran between 2008 and 2010.
In this case-control study, samples were collected using a convenience sampling method. We genotyped 164 HCV patients and 169 healthy controls for 3 SNPs in the TGF-β1 gene (-509 promoter, codon 10, and codon 25). We determined the SNP genotypes by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. To confirm the PCR-RFLP genotyping results, 10% of the samples were re-genotyped using a direct sequencing method.
There were no significant differences in the allelic frequency distribution of SNPs at -509 C/T, +869 C/T, or +915 G/C between HCV patients and the healthy controls. Genotyping results for all three polymorphic sites were similar with no statistically significant differences between the groups.
Most of the Iranian patients (over 85%), both healthy controls and HCV patients, had the GG genotype at the +915 G/C position, resulting in a high level of TGF-β1 production. Therefore, we concluded that the SNPs investigated by us cannot be considered as prognostic factors for HCV infection in our population, despite being reported as prognostic markers in other populations. Moreover, there is a possibility that most of the population is susceptible to HCV infection.
慢性丙型肝炎感染由丙型肝炎病毒（HCV）引起，其临床并发症包括肝硬化、肝衰竭和肝细胞癌。转化生长因子-β1（TGF-β1）是细胞生长与分化、血管生成、细胞外基质形成、免疫反应调节以及癌症发生和发展过程中的一种重要细胞因子。
本研究旨在调查2008年至2010年间转诊至伊朗德黑兰塔莱加尼医院的患者中，TGF-β1单核苷酸多态性（SNP）与慢性HCV感染之间的关系。
在这项病例对照研究中，采用便利抽样方法收集样本。我们对164例HCV患者和169例健康对照的TGF-β1基因中的3个SNP（-509启动子、密码子10和密码子25）进行基因分型。我们使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法确定SNP基因型。为了确认PCR-RFLP基因分型结果，10%的样本使用直接测序法重新进行基因分型。
HCV患者与健康对照之间，-509 C/T、+869 C/T或+915 G/C处SNP的等位基因频率分布无显著差异。所有三个多态性位点的基因分型结果相似，组间无统计学显著差异。
大多数伊朗患者（超过85%），包括健康对照和HCV患者，在+915 G/C位置具有GG基因型，导致TGF-β1产生水平较高。因此，我们得出结论，尽管在其他人群中这些SNP被报道为预后标志物，但我们所研究的SNP不能被视为我们人群中HCV感染的预后因素。此外，很有可能大多数人群易感染HCV。
