Kurihara K, Sukigara M, Toyoshima Y
Biochim Biophys Acta. 1979 Jul 10;547(1):117-26. doi: 10.1016/0005-2728(79)90100-2.
Photosensitivity of dispersion of phosphatidylcholine bilayer liposomes containing purified chlorophyll alpha was examined. The reduction of Cu(II) in the solution outside liposomes was observed upon illumination with visible light under anaerobic condition by means of ESR. The rate of photoreduction was significantly increased by a reductant, potassium ascorbate, localized in the solution of the opposite side of the membrane. The aciton spectrum of the reduction agreed with the absorption spectrum of chlorphyll a in the dispersion. The amount of bleach chlorophyll a was negligible compared with that of reduced (Cu(II). These facts lead to the conclusion that the potoinduced redox reactions at both the membrane-solution interfaces are coupled with each other through the bilayer of each liposome. Kinetic analysis of the reactions based on a possible reaction scheme was carried out and some of the kinetic parameters were determined.
对含有纯化叶绿素α的磷脂酰胆碱双层脂质体分散体的光敏性进行了研究。在厌氧条件下，通过电子自旋共振（ESR）观察到，用可见光照射时，脂质体外溶液中的Cu（II）会减少。位于膜另一侧溶液中的还原剂抗坏血酸钾显著提高了光还原速率。还原作用的作用光谱与分散体中叶绿素a的吸收光谱一致。与还原的（Cu（II））相比，漂白叶绿素a的量可以忽略不计。这些事实得出的结论是，每个脂质体双层膜在膜-溶液界面处的光诱导氧化还原反应相互耦合。基于可能的反应方案对反应进行了动力学分析，并确定了一些动力学参数。
