Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Geisinger Medical Center, Danville, Pennsylvania, USA.
Fertil Steril. 2012 May;97(5):1158-64.e1-4. doi: 10.1016/j.fertnstert.2012.02.001. Epub 2012 Feb 22.
To identify molecular markers associated with adhesion and normal peritoneal tissue using microarray expression profiling.
Comparative study.
University hospital.
PATIENT(S): Five premenopausal women.
INTERVENTION(S): Adhesion and normal peritoneal tissue samples were obtained from premenopausal women. Ribonucleic acid was extracted using standard protocols and processed for hybridization to Affymetrix Whole Transcript Human Gene Expression Chips. Microarray data were obtained from five different patients, each with adhesion tissue and normal peritoneal samples. Real-time polymerase chain reaction was performed for confirmation using standard protocols.
MAIN OUTCOME MEASURE(S): Gene expression in postoperative adhesion and normal peritoneal tissues.
RESULT(S): A total of 1,263 genes were differentially expressed between adhesion and normal tissues. One hundred seventy-three genes were found to be up-regulated and 56 genes were down-regulated in the adhesion tissues compared with normal peritoneal tissues. The genes were sorted into functional categories according to Gene Ontology annotations. Twenty-six up-regulated genes and 11 down-regulated genes were identified with functions potentially relevant to the pathophysiology of postoperative adhesions. We evaluated and confirmed expression of 12 of these specific genes via polymerase chain reaction.
CONCLUSION(S): The pathogenesis, natural history, and optimal treatment of postoperative adhesive disease remains unanswered. Microarray analysis of adhesions identified specific genes with increased and decreased expression when compared with normal peritoneum. Knowledge of these genes and ontologic pathways with altered expression provide targets for new therapies to treat patients who have or are at risk for postoperative adhesions.
使用基因芯片表达谱分析鉴定与粘连和正常腹膜组织相关的分子标志物。
对比研究。
大学医院。
5 名绝经前妇女。
从绝经前妇女中获取粘连组织和正常腹膜组织样本。采用标准方案提取核糖核酸并进行杂交，以 Affymetrix Whole Transcript Human Gene Expression Chips。从 5 名不同的患者中获得微阵列数据，每位患者均有粘连组织和正常腹膜样本。采用标准方案进行实时聚合酶链反应以进行确认。
术后粘连组织和正常腹膜组织中的基因表达。
粘连组织和正常组织之间共有 1263 个基因差异表达。与正常腹膜组织相比，粘连组织中发现有 173 个基因上调，56 个基因下调。根据基因本体论注释，这些基因被分类为功能类别。有 26 个上调基因和 11 个下调基因与术后粘连的病理生理学有潜在相关性。我们通过聚合酶链反应评估并证实了其中 12 个特定基因的表达。
术后粘连病的发病机制、自然病史和最佳治疗方法仍未得到解答。粘连的基因芯片分析确定了与正常腹膜相比表达增加和减少的特定基因。这些基因和改变表达的本体途径的知识为治疗有或有发生术后粘连风险的患者提供了新疗法的靶点。
