School of Medicine, Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, China.
Biomed Environ Sci. 2011 Dec;24(6):608-16. doi: 10.3967/0895-3988.2011.06.004.
To create transgenic mice expressing hamster- and human-PRNP as a model for understanding the physiological function and pathology of prion protein (PrP), as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies (TSEs).
Hamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin (H & E) staining and immunohistochemical (IHC) methods.
Integrated PRNP copy number in various mouse lines was 53 (Tg-haPrP1), 18 (Tg-huPrP1), 3 (Tg-huPrP2), and 16 (Tg-huPrP5), respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs.
We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.
构建表达仓鼠和人朊蛋白的转基因小鼠，以了解朊蛋白（PrP）的生理功能和病理学，以及传染性海绵状脑病（TSE）跨物种传播的机制。
通过常规方法建立仓鼠和人朊蛋白转基因小鼠。通过实时 PCR 定位整合 PRNP 的拷贝数。通过半定量 RT-PCR、实时 RT-PCR 和 Western blot 分析确定 PRNP mRNA 和蛋白水平。通过苏木精和伊红（H & E）染色和免疫组织化学（IHC）方法对转基因小鼠进行组织学分析。
各小鼠系的整合 PRNP 拷贝数分别为 53（Tg-haPrP1）、18（Tg-huPrP1）、3（Tg-huPrP2）和 16（Tg-huPrP5）。外源性 PrPs 在转录和翻译水平上均有表达。组织学检测未发现脑或其他器官的任何异常。
我们已经建立了一个仓鼠-PRNP 转基因小鼠系和三个人类-PRNP 转基因小鼠系。这四个转基因小鼠系为进一步的研究提供了理想的模型。
