College of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, PR China.
Talanta. 2012 Mar 15;91:83-7. doi: 10.1016/j.talanta.2012.01.022. Epub 2012 Jan 18.
A rapid and efficient capillary zone electrophoresis (CZE) method with ultraviolet detection has been successfully developed for the determination of puerarin in Xinkeshu capsules and biological samples. The optimal CZE conditions were determined to be 70 mM H(3)BO(3)-Na(2)B(4)O(7), pH 9.2, injection time 5s, applied voltage 25 kV, working temperature 25°C and detection wavelength 254 nm. Under these conditions, a linear range from 17.3 to 138 μg/mL with the correlation coefficient of 0.9998 and limit of detection of 34.6 ng/mL (S/N=3) for puerarin were obtained. The relative standard deviation of the migration time and peak area of puerarin were 0.23-0.91, 0.43-1.62, 0.10-0.64 and 0.64-1.32% for intra-day and inter-day analyses, respectively. Satisfactory results were obtained for recovery and repeatability. The proposed method was successfully applied to analyze the puerarin content in Xinkeshu capsules and some biological samples with good results. It is suitable for applications in pharmaceutical industries for quality control and in clinical laboratories for therapeutic drug monitoring purposes.
一种快速高效的毛细管区带电泳（CZE）方法，结合紫外检测，已成功应用于测定心可舒胶囊和生物样品中的葛根素。确定的最佳 CZE 条件为 70mM H（3）BO（3）-Na（2）B（4）O（7），pH9.2，进样时间 5s，外加电压 25kV，工作温度 25°C，检测波长 254nm。在此条件下，葛根素的线性范围为 17.3-138μg/mL，相关系数为 0.9998，检测限为 34.6ng/mL（S/N=3）。葛根素的迁移时间和峰面积的日内和日间相对标准偏差分别为 0.23-0.91%、0.43-1.62%、0.10-0.64%和 0.64-1.32%。回收率和重复性的结果令人满意。该方法成功应用于分析心可舒胶囊和一些生物样品中的葛根素含量，结果良好。该方法适用于制药行业的质量控制和临床实验室的治疗药物监测。
