Singh Azad, Bedekar Megha Kadam, Sharma Rakesh, Sarkhel Bikash Chandra, Singh Sanjeev, Jain Sudhir Kumar
MPPCVV Animal Biotechnology Centre, Jabalpur, India.
Acta Vet Hung. 2012 Mar;60(1):165-74. doi: 10.1556/AVet.2012.014.
In order to detect infectious bursal disease virus (IBDV), bursal tissue was collected from 10 IBD-suspected birds from a 30-day-old, IBDV-vaccinated commercial broiler chicken flock of 2000 birds exhibiting clinical signs suggestive of infectious bursal disease (IBD). The presence of IBDV was confirmed by partial amplification of the VP2 gene by reverse transcription and polymerase chain reaction. Isolates were identified as very virulent strains of IBDV (vvIBDV) by nucleotide sequence analysis. The comparison of the VP2 nucleotide sequences among the isolates revealed the presence of single-nucleotide polymorphisms in the VP2 gene of IBDV in the same flock. The comparative analysis indicated that these viruses were genetically close to the vvIBDVs previously detected in India. Our analysis provided information about the existence of vvIBDV in Central India.
为检测传染性法氏囊病病毒（IBDV），从一个有2000只30日龄IBDV疫苗接种的商品肉鸡群中，选取了10只表现出传染性法氏囊病（IBD）临床症状的疑似IBD禽类，采集其法氏囊组织。通过逆转录和聚合酶链反应对VP2基因进行部分扩增，证实了IBDV的存在。通过核苷酸序列分析，分离株被鉴定为IBDV的超强毒株（vvIBDV）。对分离株之间VP2核苷酸序列的比较显示，同一鸡群中IBDV的VP2基因存在单核苷酸多态性。比较分析表明，这些病毒在基因上与先前在印度检测到的vvIBDV接近。我们的分析提供了关于印度中部存在vvIBDV的信息。
