Chemical and Materials Engineering, University of Kentucky, Lexington, KY, USA.
Acta Biomater. 2012 Jul;8(6):2096-103. doi: 10.1016/j.actbio.2012.02.012. Epub 2012 Feb 22.
Antioxidant enzymes for the treatment of oxidative stress-related diseases remain a highly promising therapeutic approach. As poor localization and stability have been the greatest challenges to their clinical translation, a variety of nanocarrier systems have been developed to directly address these limitations. In most cases, there has been a trade-off between the delivered mass of enzyme loaded and the carrier's ability to protect the enzyme from proteolytic degradation. One potential method of overcoming this limitation is the use of ordered mesoporous silica materials as potential antioxidant enzyme nanocarriers. The present study compared the loading, activity and retention activity of an anti-oxidant enzyme, catalase, on four engineered mesoporous silica types: non-porous silica particles, spherical silica particles with radially oriented pores and hollow spherical silica particles with pores oriented either parallel to the hollow core or expanded, interconnected bimodal pores. All these silica types, except non-porous silica, displayed potential for effective catalase loading and protection against the proteolytic enzyme, pronase. Hollow particles with interconnected pores exhibit protein loading of up to 50 wt.% carrier mass, while still maintaining significant protection against proteolysis.
抗氧化酶治疗与氧化应激相关的疾病仍然是一种极具前景的治疗方法。由于定位和稳定性差一直是其临床转化的最大挑战，因此已经开发了各种纳米载体系统来直接解决这些限制。在大多数情况下，负载的酶的量与载体保护酶免受蛋白水解降解的能力之间存在权衡。克服这一限制的一种潜在方法是使用有序介孔硅材料作为潜在的抗氧化酶纳米载体。本研究比较了四种工程介孔硅材料（无孔硅颗粒、具有径向取向孔的球形硅颗粒和具有平行于空心核或扩展的、相互连通的双模态孔的取向孔的空心球形硅颗粒）对抗氧化酶（过氧化氢酶）的负载、活性和保留活性的影响。除了无孔硅之外，所有这些硅类型都显示出有效负载过氧化氢酶的潜力，并能防止蛋白水解酶胰蛋白酶的降解。具有相互连通孔的空心颗粒的蛋白负载量高达载体质量的 50wt%，同时仍能保持对蛋白水解的显著保护作用。
