Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomiya, Tochigi 321-8505, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 2012 May;162(1-3):44-50. doi: 10.1016/j.cbpb.2012.02.001. Epub 2012 Feb 17.
Through BLAST search of the genomic database of Bombyx mori, we found a clone, BmorCPG11, which has only putative BR-C binding sites in the 2 kb upstream region. Both BmorCPG11 and BR-Z2 were expressed in the cephalic region of the epidermis, differently from BR-Z1 and BR-Z4. BR-Z2 transcripts increased by the addition of 20-hydroxyl-ecdysone (20E), which was slightly inhibited by cycloheximide. BmorCPG11 expression was also induced by the addition of 20E, which was inhibited by cycloheximide. Both BmorCPG11 and BR-Z2 were induced by the 20E pulse treatment, but they were inhibited by the addition of cycloheximide. Both genes showed similar expression pattern in wing discs during the developmental stage and in vitro ecdysone responsiveness, and both showed relatedness. The result of a reporter assay demonstrated the strong relatedness of BmorCPG11 and BR-Z2. The expression profiles of BmorCPG11 and BR-Z2 are different from those of BMWCP5 and βFTZ-F1. The present findings showed different regulation of cuticular protein genes by ecdysone-responsive transcription factors at the pre-pupal stage of wing discs of B. mori.
通过对家蚕基因组数据库的 BLAST 搜索，我们发现了一个克隆，BmorCPG11，其 2 kb 上游区域只有假定的 BR-C 结合位点。BmorCPG11 和 BR-Z2 均在表皮的头部区域表达，与 BR-Z1 和 BR-Z4 不同。BR-Z2 转录物通过添加 20-羟基蜕皮激素（20E）而增加，该增加被环己酰亚胺轻微抑制。BmorCPG11 的表达也被 20E 的添加诱导，该添加被环己酰亚胺抑制。BmorCPG11 和 BR-Z2 均被 20E 脉冲处理诱导，但被环己酰亚胺添加抑制。这两个基因在发育阶段的翅膀盘和体外蜕皮激素反应性中表现出相似的表达模式，并且具有相关性。报告基因测定的结果表明 BmorCPG11 和 BR-Z2 之间具有很强的相关性。BmorCPG11 和 BR-Z2 的表达谱与 BMWCP5 和βFTZ-F1 不同。本研究结果表明，在 B. mori 翅膀盘的预蛹期，蜕皮激素反应性转录因子对表皮蛋白基因的调控不同。
