Dept. of Biotechnology, Indian Institute of Integrative Medicine (CSIR), Srinagar-190005, India.
Int Immunopharmacol. 2012 Apr;12(4):626-34. doi: 10.1016/j.intimp.2012.02.001. Epub 2012 Feb 21.
In order to evaluate the role of ethyl acetate fraction (PNRS-EtOAC) obtained from the Prunus cerasus fruit in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PNRS-EtOAC (25-100 mg/kg) stimulated the IgM and IgG titre expressed in the form of hemagglutination antibody (HA) titre. Further, it elicited a dose related increase in the delayed type hypersensitivity reaction (DTH) after 24 and 48 h in BALB/c mice. Besides augmenting the humoral and cell mediated immune response, the concentration of cytokines (IFN-γ, IL-4, and TNF-α) in serum with respect to T cell interactions, i.e. the proliferation of lymphocytes were significantly increased at 50 mg/kg compared with the control. The results in these studies demonstrated the immunostimulatory effect of PNRS-EtOAC in a dose-dependent manner with respect to the macrophage activation possibly expressing the phagocytosis and nitrite production by the enhancement of TNF-α production as a mode of action.
为了评估从欧洲甜樱桃果实中获得的乙酸乙酯级分（PNRS-EtOAC）在调节免疫反应中的作用，使用一系列体内试验进行了详细研究。PNRS-EtOAC（25-100mg/kg）的口服给药刺激了以血凝抗体（HA）效价形式表达的 IgM 和 IgG 滴度。此外，它在 BALB/c 小鼠中在 24 和 48 小时后引起了与剂量相关的迟发型超敏反应（DTH）增加。除了增强体液和细胞介导的免疫反应外，与 T 细胞相互作用（即淋巴细胞增殖）相关的血清细胞因子（IFN-γ、IL-4 和 TNF-α）的浓度在 50mg/kg 时与对照组相比显著增加。这些研究的结果表明，PNRS-EtOAC 具有剂量依赖性的免疫刺激作用，可能通过增强 TNF-α 产生作为作用模式来表达巨噬细胞激活，从而实现吞噬作用和亚硝酸盐产生。
