Department of Immunology, Genentech, South San Francisco, California, USA.
Nat Immunol. 2012 Feb 26;13(4):396-404. doi: 10.1038/ni.2256.
Immunoglobulin E (IgE) antibodies are pathogenic in asthma and allergic diseases, but the in vivo biology of IgE-producing (IgE(+)) cells is poorly understood. A model of the differentiation of IgE(+) B cells proposes that IgE(+) cells develop through a germinal-center IgG1(+) intermediate and that IgE memory resides in the compartment of IgG1(+) memory B cells. Here we have used a reporter mouse expressing green fluorescent protein associated with membrane IgE transcripts (IgE-GFP) to assess in vivo IgE responses. In contrast to the IgG1-centered model of IgE switching and memory, we found that IgE(+) cells developed through a germinal-center IgE(+) intermediate to form IgE(+) memory B cells and plasma cells. Our studies delineate a new model for the in vivo biology of IgE switching and memory.
免疫球蛋白 E(IgE)抗体在哮喘和过敏性疾病中具有致病性,但 IgE 产生(IgE(+))细胞的体内生物学特性仍知之甚少。一种 IgE(+)B 细胞分化的模型提出,IgE(+)细胞通过生发中心 IgG1(+)中间产物发展而来,IgE 记忆存在于 IgG1(+)记忆 B 细胞区室中。在这里,我们使用表达与膜 IgE 转录本相关的绿色荧光蛋白的报告小鼠(IgE-GFP)来评估体内 IgE 反应。与 IgG1 为中心的 IgE 转换和记忆模型相反,我们发现 IgE(+)细胞通过生发中心 IgE(+)中间产物发展为 IgE(+)记忆 B 细胞和浆细胞。我们的研究描绘了 IgE 转换和记忆的体内生物学的新模型。