Laboratory for Lymphocyte Differentiation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan.
Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12192-7. doi: 10.1073/pnas.1005443107. Epub 2010 Jun 14.
It has long been presumed that after leaving the germinal centers (GCs), memory B cells colonize the marginal zone or join the recirculating pool. Here we demonstrate the preferential localization of nitrophenol-chicken gamma-globulin-induced CD38(+)IgG1(+) memory B cells adjacent to contracted GCs in the spleen. The memory B cells in this region proliferated after secondary immunization, a response that was abolished by depletion of CD4(+) T cells. We also found that these IgG1(+) memory B cells could present antigen on their surface, and that this activity was required for their activation. These results implicate this peri-GC region as an important site for survival and reactivation of memory B cells.
长期以来，人们一直认为，生发中心（GC）离开后，记忆 B 细胞定殖于边缘区或加入循环池。在这里，我们证明了硝基酚鸡γ球蛋白诱导的 CD38(+)IgG1(+)记忆 B 细胞优先定位于脾脏中收缩的 GC 附近。该区域的记忆 B 细胞在二次免疫后增殖，该反应被耗尽 CD4(+)T 细胞所消除。我们还发现这些 IgG1(+)记忆 B 细胞可以在其表面呈递抗原，并且这种活性是其激活所必需的。这些结果表明，GC 周围区域是记忆 B 细胞存活和重新激活的重要部位。
