Artsukevich I M
Biokhimiia. 1979 Mar;44(3):543-7.
The nucleotide specificity of thiamine pyrophosphokinase from rat liver was studied. The enzyme was found to possess a sufficiently wide substrate specificity. Any of the nucleotides can be a donor of the pyrophosphate groups for TDP biosynthesis at two pH optima of the enzyme in the T-kinase reaction under the Mg2++/NTP optimal ratio. The minimal requirement for the substrate structure allowing to predict the position of the split nucleotide phosphoester bond was postulated.
对大鼠肝脏硫胺素焦磷酸激酶的核苷酸特异性进行了研究。发现该酶具有足够广泛的底物特异性。在Mg2++/NTP最佳比例下的T激酶反应中，在该酶的两个最适pH值条件下，任何一种核苷酸都可以作为焦磷酸基团供体用于TDP生物合成。推测了底物结构允许预测断裂的核苷酸磷酸酯键位置的最低要求。
