Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province, Henan Provincial Key Laboratory of Fruit Plant and Cucurbit, Horticulture College of Henan Agricultural University, Zhengzhou 450002, Henan, China.
Biochem Genet. 2012 Aug;50(7-8):565-78. doi: 10.1007/s10528-012-9501-9. Epub 2012 Feb 25.
Heat shock proteins (Hsps) are a family of highly conserved proteins present in all organisms. They mediate a range of cytoprotective functions as molecular chaperones and are recently reported to regulate the immune response. Using suppression subtractive hybridization, we isolated and characterized a cucumber cDNA, designated CsHsp45.9, which encodes a putative heat shock protein of 45.9 kDa protein, containing three conserved DnaJ domains belonging to the Type I Hsp40 family. Real-time quantitative RT-PCR analysis revealed that CsHsp45.9 was significantly induced in cucumber leaves inoculated with downy mildew (Pseudoperonospora cubensis) in this incompatible interaction. Gene expression was also strongly up-regulated by various abiotic stresses. CsHsp45.9 was mainly expressed in flowers with a flower-specific, stamen- and pistil-predominant expression pattern. This suggests that CsHsp45.9 harbors broad-spectrum responses to both biotic and abiotic stresses and may play a role in downy mildew resistance in cucumber.
热休克蛋白（Hsps）是存在于所有生物体中的高度保守的蛋白质家族。它们作为分子伴侣介导一系列细胞保护功能，最近有报道称它们可以调节免疫反应。我们使用抑制性消减杂交分离并鉴定了一个黄瓜 cDNA，命名为 CsHsp45.9，它编码一个推定的 45.9 kDa 蛋白的热休克蛋白，含有三个保守的 DnaJ 结构域，属于 I 型 Hsp40 家族。实时定量 RT-PCR 分析表明，在黄瓜叶片与霜霉病（Pseudoperonospora cubensis）的非亲和互作中，CsHsp45.9 显著诱导。基因表达也被各种非生物胁迫强烈上调。CsHsp45.9 主要在花朵中表达，具有花特异性、雄蕊和雌蕊优势表达模式。这表明 CsHsp45.9 对生物和非生物胁迫具有广谱反应，可能在黄瓜霜霉病抗性中发挥作用。
