College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China.
Int J Mol Sci. 2018 Jul 3;19(7):1950. doi: 10.3390/ijms19071950.
Blast disease is one of the major rice diseases, and causes nearly 30% annual yield loss worldwide. Resistance genes that have been cloned, however, are effective only against specific strains. In cultivation practice, broad-spectrum resistance to various strains is highly valuable, and requires researchers to investigate the basal defense responses that are effective for diverse types of pathogens. In this study, we took a quantitative proteomic approach and identified 634 rice proteins responsive to infections by both strains Guy11 and JS153. These two strains have distinct pathogenesis mechanisms. Therefore, the common responding proteins represent conserved basal defense to a broad spectrum of blast pathogens. Gene ontology analysis indicates that the “responding to stimulus” biological process is explicitly enriched, among which the proteins responding to oxidative stress and biotic stress are the most prominent. These analyses led to the discoveries of OsPRX59 and OsPRX62 that are robust callose inducers, and OsHSP81 that is capable of inducing both ROS production and callose deposition. The identified rice proteins and biological processes may represent a conserved rice innate immune machinery that is of great value for breeding broad-spectrum resistant rice in the future.
稻瘟病是主要的水稻病害之一，全球每年因此损失近 30%的产量。然而，已克隆的抗性基因仅对特定菌株有效。在栽培实践中，对各种菌株的广谱抗性极具价值，这需要研究人员研究对不同类型病原体有效的基础防御反应。在这项研究中，我们采用定量蛋白质组学方法，鉴定出了对菌株 Guy11 和 JS153 感染均有反应的 634 种水稻蛋白。这两个菌株具有不同的发病机制。因此，共同反应的蛋白代表了对广谱稻瘟病菌的保守基础防御。GO 分析表明，“对刺激的反应”这一生物学过程明显富集，其中对氧化应激和生物胁迫有反应的蛋白最为突出。这些分析导致了 OsPRX59 和 OsPRX62 的发现，它们是强有力的胼胝质诱导剂，而 OsHSP81 能够诱导 ROS 产生和胼胝质沉积。所鉴定的水稻蛋白和生物学过程可能代表了一种保守的水稻先天免疫机制，这对于未来培育广谱抗性水稻具有重要价值。
