Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
J Microbiol. 2012 Feb;50(1):50-7. doi: 10.1007/s12275-012-1417-x. Epub 2012 Feb 27.
Endophytic actinobacterial diversity in the native herbaceous plant species of Korea was analyzed using a culture-based approach. Sixty one actinobacterial strains were isolated, and assigned to 15 genera based on 16S rRNA gene analysis. The members of the genus Streptomyces comprised 45.9% of the total isolates, followed by Micromonospora (18.8%), Rhodococcus (6.6%), Microbispora (4.9%), and Micrococcus (4.9%). Other minor constituents included members of Microbacterium, Streptacidiphilus, Arthrobacter, Dietzia, Kitasatospora, Herbiconiux, Mycobacterium, Nocardia, Rathayibacter, and Tsukamurella. Among the isolates, 65.6% exhibited at least one hydrolytic enzyme activity out of four, and 45.9% exhibited antagonistic activity against at least one fungal pathogen out of five, thus demonstrating that endophytic actinobacteria can be an important source of bioactive compounds. Notably, most strains of Streptomyces proved active for both enzymatic and antagonistic activities.
采用基于培养的方法分析了韩国本土草本植物种内的内生放线菌多样性。从 61 株放线菌菌株中分离得到 15 个属，基于 16S rRNA 基因分析进行分类。链霉菌属的成员占总分离株的 45.9%，其次是微单胞菌属（18.8%）、红球菌属（6.6%）、小单孢菌属（4.9%）和微球菌属（4.9%）。其他较少的成分包括微杆菌属、链霉菌酸嗜热菌属、节杆菌属、迪茨氏菌属、北里孢菌属、草间内生球菌属、分枝杆菌属、诺卡氏菌属、拉氏菌属和土生古菌属。在这些分离株中，65.6%的菌株至少表现出四种水解酶活性中的一种，45.9%的菌株至少对五种真菌病原体中的一种表现出拮抗活性，因此表明内生放线菌可以成为生物活性化合物的重要来源。值得注意的是，大多数链霉菌菌株在酶活性和拮抗活性方面都表现出较高的活性。
