Hu Ze-Lan, Huang Ying, Tao Xiao-Rong, Qi Zheng-Han, Chen Jia-Yin, Ding Yu-Qiang
Key Laboratory of Arrhythmias, Ministry of Education, Tongji University School of Medicine, Shanghai 200092, China.
Genesis. 2012 Jul;50(7):552-60. doi: 10.1002/dvg.22020. Epub 2012 Apr 3.
Prrxl1-CreER(T2) transgenic mice expressing tamoxifen-inducible Cre recombinase were generated by modifying a Prrxl1-containing BAC clone. Cre recombination activity was examined in Prrxl1-CreER(T2); Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a single dose of tamoxifen at embryonic day (E) 9.5 or E12.5, and X-gal staining was performed 2 days later. Strong X-gal staining was observed in the somatosensory ganglia (e.g., dorsal root and trigeminal ganglia) and the first central sites for processing somatosensory information (e.g., spinal dorsal horn and trigeminal nerve-associated nuclei). When tamoxifen was administered at postnatal day (P) 20 or in adulthood (P120), strong Cre recombination activity was present in the primary somatosensory ganglia, while weak Cre recombination activity was found in the spinal dorsal horn, mesencephalic trigeminal nucleus, principal sensory trigeminal nucleus, and spinal trigeminal nucleus. This mouse line provides a useful tool for exploring genes' functions in the somatosensory system in a time-controlled way.
通过修饰含Prrxl1的细菌人工染色体（BAC）克隆，构建了表达他莫昔芬诱导型Cre重组酶的Prrxl1-CreER(T2)转基因小鼠。在不同胚胎期和出生后阶段的Prrxl1-CreER(T2); Rosa26报告基因小鼠中检测Cre重组活性。怀孕小鼠在胚胎第9.5天（E9.5）或E12.5接受单剂量他莫昔芬处理，2天后进行X-gal染色。在躯体感觉神经节（如背根神经节和三叉神经节）以及处理躯体感觉信息的首个中枢位点（如脊髓背角和三叉神经相关核团）观察到强烈的X-gal染色。当在出生后第20天（P20）或成年期（P120）给予他莫昔芬时，在初级躯体感觉神经节中存在强烈的Cre重组活性，而在脊髓背角、中脑三叉神经核、三叉神经主感觉核和脊髓三叉神经核中发现较弱的Cre重组活性。该小鼠品系为以时间可控的方式探索躯体感觉系统中基因的功能提供了有用的工具。
