Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan, ROC.
J Hazard Mater. 2012 Apr 30;213-214:341-6. doi: 10.1016/j.jhazmat.2012.02.009. Epub 2012 Feb 10.
Polybrominated diphenyl ethers (PBDEs) are widely used flame retardant additives for many plastic and electronic products. Owing to their ubiquitous distribution in the environment, multiple toxicity to humans, and increasing accumulation in the environment, the fate of PBDEs is of serious concern for public safety. In this study, the degradation of 4-monobrominated diphenyl ether (BDE-3) in anaerobic sludge and the effect of carbon source addition were investigated. BDE-3 can be degraded by two different anaerobic sludge samples. The by-products, diphenyl ether (DE) and bromide ions, were monitored, indicating the reaction of debromination within these anaerobic samples. Co-metabolism with glucose facilitated BDE-3 biodegradation in terms of kinetics and efficiency in the Jhongsing sludge. Through the pattern of amplified 16S rRNA gene fragments in denatured gradient gel electrophoresis (DGGE), the composition of the microbial community was analyzed. Most of the predominant microbes were novel species. The fragments enriched in BDE-3-degrading anaerobic sludge samples are presumably Clostridium sp. This enrichment coincides with the H(2) gas generation and the facilitation of debromination during the degradation process. Findings of this study provide better understanding of the biodegradation of brominated DEs and can facilitate the prediction of the fate of PBDEs in the environment.
多溴联苯醚（PBDEs）是许多塑料和电子产品中广泛使用的阻燃添加剂。由于其在环境中广泛分布、对人类的多种毒性以及在环境中的不断积累，PBDEs 的命运引起了公众安全的严重关注。本研究考察了 4-单溴联苯醚（BDE-3）在厌氧污泥中的降解以及添加碳源的影响。两种不同的厌氧污泥样品都可以降解 BDE-3。监测到的副产物二苯醚（DE）和溴化物离子表明，这些厌氧样品中存在脱溴反应。在钟兴污泥中，葡萄糖的共代谢促进了 BDE-3 的生物降解，无论是在动力学方面还是在效率方面。通过变性梯度凝胶电泳（DGGE）中扩增的 16S rRNA 基因片段模式分析了微生物群落的组成。大多数主要微生物是新型物种。在富含 BDE-3 的厌氧污泥样品中富集的片段可能是梭菌属（Clostridium sp.）。这种富集与降解过程中 H2 气体的产生和脱溴的促进相一致。本研究的结果提供了对溴化 DE 生物降解的更好理解，并有助于预测 PBDEs 在环境中的命运。
