Department of Biological Sciences, Kangwon National University, Chuncheon 200-701, Korea.
J Microbiol Biotechnol. 2012 Feb;22(2):239-43. doi: 10.4014/jmb.1107.07050.
Degradation of butylbenzyl phthalate (BBP) by the white rot fungus Pleurotus ostreatus and the activities of some degrading enzymes were examined in two different media containing 100 mg/l of the compound. P. ostreatus pregrown for 7 days in complex YMG medium was able to completely degrade BBP within an additional 24 h but degraded only 35 mg/l of BBP in 5 days of incubation in minimal medium. Fungal cell mass in the culture in YMG medium was higher in the presence than in the absence of BBP. The esterase activity of the fungal culture in YMG medium was higher than that in minimal medium and increased with the addition of BBP. On the contrary, laccase activity was higher in minimal medium and it did not increase upon the addition of BBP. General peroxidase activity increased for a few days after the addition of BBP to both media. The degradation of BBP and its metabolites by P. ostreatus thus may be attributed mostly to esterase rather than lignin-degrading laccase. In addition, the activities of the enzymes involved in BBP degradation and their changes varied significantly in the different media and culture conditions.
研究了白腐菌糙皮侧耳（Pleurotus ostreatus）在两种不同的培养基中对邻苯二甲酸丁基苄基酯（BBP）的降解及其一些降解酶的活性，这两种培养基中均含有 100mg/L 的该化合物。在含有复杂 YMG 培养基中预培养 7 天的糙皮侧耳能够在另外 24 小时内完全降解 BBP，但在最小培养基中培养 5 天仅能降解 35mg/L 的 BBP。在 YMG 培养基中存在 BBP 时，真菌细胞质量高于不存在 BBP 时。YMG 培养基中真菌培养物的酯酶活性高于最小培养基中的酯酶活性，且随着 BBP 的添加而增加。相反，漆酶活性在最小培养基中更高，而添加 BBP 后不会增加。在向两种培养基中添加 BBP 后的几天内，过氧化物酶的总活性增加。因此，邻苯二甲酸丁基苄基酯的降解及其代谢物可能主要归因于酯酶而不是木质素降解漆酶。此外，参与 BBP 降解的酶的活性及其在不同培养基和培养条件下的变化有很大差异。
