Department of General Biophysics, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.
J Physiol Biochem. 2012 Sep;68(3):447-54. doi: 10.1007/s13105-012-0158-y. Epub 2012 Feb 28.
Biogenic amines and polyamines participate in all vital organism functions, their levels being important function determinants. Studies were performed to check whether repeated administration of poly(propylene imine) (PPI) dendrimers, synthetic macromolecules with diaminobutane core, and peripheral primary amine groups, may influence the endogenous level of amines, as represented by the two of them: spermidine, a natural derivative of diaminobutane, and histamine. The experiment was carried out on Wistar rats. Fourth generation PPI dendrimer, as well as maltotriose-modified fourth generation PPI dendrimers with (a) cationic open sugar shell and (b) neutral dense sugar shell that possess a higher biocompatibility, was used. Applying the combination of column chromatography on Cellex P and spectrofluorimetric assays of o-phthaldialdehyde, the final amine condensation products were employed to analyze tissue spermidine and histamine outside the central nervous system. Furthermore, radioenzymatic assay was used to measure histamine levels in the brain. The obtained results indicate that in some tissues, the endogenous concentrations of histamine and spermidine may be affected by dendrimers depending on their dose and type of dendrimers.
生物胺和多胺参与所有生命有机体的功能，其水平是重要的功能决定因素。本研究旨在检查重复给予具有二氨基丁烷核心和周围伯胺基团的合成大分子多（丙稀亚胺）（PPI）树枝状聚合物是否会影响生物胺的内源性水平，以两种生物胺作为代表：亚精胺，二氨基丁烷的天然衍生物，和组胺。该实验在 Wistar 大鼠上进行。使用了第四代 PPI 树枝状聚合物以及麦芽糖三糖修饰的第四代 PPI 树枝状聚合物（a）具有阳离子开糖壳和（b）具有更高生物相容性的中性密糖壳。应用 Cellex P 柱层析和邻苯二醛分光荧光法分析组织外亚精胺和组织外组胺，最后用酶联免疫吸附法测量脑组织中的组胺水平。结果表明，在某些组织中，内源性组胺和亚精胺的浓度可能会受到树枝状聚合物的影响，这取决于其剂量和类型。
