Li Xiao-Dong, Yang Li, Xu Shi-Qian, Li Jian-Guo, Cheng Zhi-Hui, Dang Jian-Zhang
School of Applied Biological Engineering, Shenzhen Polytechnic, Shenzhen 518055, China.
Zhong Yao Cai. 2011 Oct;34(10):1544-8.
To extract the essential oils from the Seedlings, the Aseptic Seedlings and the Tissue Culture Seedlings of Thymus vulgaris and analyze their chemical components and the relative contents.
The essential oils were extracted by steam distillation, the chemical components and the relative contents were identified and analyzed by gas chromatography-mass spectrometry (GC/MS) and peak area normalization method.
The main chemical components of essential oil in these three samples had no significant difference, they all contained the main components of essential oil in Thymus vulgaris: Thymol, Carvacrol, o-Cymene, gamma-Terpinene, Caryophyllene et al. and only had a slight difference in the relative content.
This study provides important theoretical foundation and data reference for further study on production of essential oil in thyme by tissue culture technology.
提取百里香实生苗、无菌苗和组培苗的挥发油，并分析其化学成分及相对含量。
采用水蒸气蒸馏法提取挥发油，利用气相色谱-质谱联用仪（GC/MS）和峰面积归一化法对其化学成分及相对含量进行鉴定与分析。
这三个样品挥发油的主要化学成分无显著差异，均含有百里香挥发油的主要成分：百里香酚、香芹酚、邻异丙基甲苯、γ-萜品烯、石竹烯等，相对含量仅存在细微差异。
本研究为进一步利用组织培养技术生产百里香挥发油提供了重要的理论依据和数据参考。
