Research School of Biology, The Australian National University, Canberra, Australia.
Photochem Photobiol. 2012 Nov-Dec;88(6):1418-27. doi: 10.1111/j.1751-1097.2012.01130.x. Epub 2012 Mar 28.
Glial cells play an important role in the maintenance of normal structure and function of the neural components of the central nervous system. The Müller cells are one of the macroglial elements in the retina and their wide-ranging roles are responsible for the protection and proper functioning of the photoreceptors. In the present study, we aimed to test the effects of pretreatment with 670 nm red light on Müller cells in the light-induced model of retinal degeneration. Adult Sprague-Dawley albino rats were treated with 670 nm red light, from an LED source prior to exposure to bright (1000 lux) continuous light for 24 h. Müller cell-specific markers were used to assess structural and functional changes in this cell type 1 week after contact with damaging light. Changes in gene (Edn2, LIF, TNF-α) and protein (S100β, Vimentin, LIF, iNOS, GS, Cyclin-D1) levels and localization were evaluated using RT-qPCR, and immunohistochemistry. Our results showed that 670 nm light pretreatment ameliorates the light-induced alterations in the expression of Müller-cell specific markers for structure, stress, metabolism and inflammation. This suggests that 670 nm light preconditioning may promote neuroprotective effects in the retina from light-induced damage, possibly through pathways regulating the roles of Müller cells in maintaining retinal homeostasis.
胶质细胞在维持中枢神经系统神经成分的正常结构和功能方面发挥着重要作用。Müller 细胞是视网膜中的一种大胶质细胞，其广泛的作用负责保护和适当运作光感受器。在本研究中，我们旨在测试 670nm 红光预处理对光诱导视网膜变性模型中 Müller 细胞的影响。成年 Sprague-Dawley 白化大鼠在暴露于明亮（1000 勒克斯）连续光 24 小时之前，用来自 LED 源的 670nm 红光进行预处理。Müller 细胞特异性标志物用于评估接触损伤性光后 1 周这种细胞类型的结构和功能变化。使用 RT-qPCR 和免疫组织化学评估基因（Edn2、LIF、TNF-α）和蛋白质（S100β、Vimentin、LIF、iNOS、GS、Cyclin-D1）水平和定位的变化。我们的结果表明，670nm 光预处理可改善光诱导的 Müller 细胞特异性标志物表达的改变，这些标志物与结构、应激、代谢和炎症有关。这表明 670nm 光预处理可能通过调节 Müller 细胞在维持视网膜内稳态中的作用的途径，促进视网膜免受光诱导损伤的神经保护作用。
