Department of Pathology, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA.
Arch Pathol Lab Med. 2012 Mar;136(3):294-300. doi: 10.5858/2011-0127-CPR.1.
Data collection and analysis of the College of American Pathologists (CAP) Interlaboratory Comparison Program (Proficiency Testing) J-Survey results provide insights into North American pretransfusion compatibility testing practices and trends.
To assess current North American manual testing practices for ABO grouping, rhesus (Rh) typing, antibody screening, and crossmatching using CAP proficiency testing data.
Analysis of the CAP Interlaboratory Comparison Program J-Survey data (2005-2010) to identify laboratory methods used for ABO grouping, Rh typing, antibody screening, and crossmatching. Data were analyzed by test method using Microsoft (Redmond, Washington) Excel software.
The method used most often in ABO grouping and Rh typing was tube testing. Many laboratories also used tube testing for antibody detection and crossmatching, but during the study period, the proportion of laboratories using gel-based methodologies increased considerably.
Most North American CAP laboratories continue to use tube methods for ABO/Rh testing. Use of gel-based methodologies increased during the past 5 years for antibody screening and crossmatching.
美国病理学家学会（CAP）实验室间比对计划（能力验证）J 调查结果的数据收集和分析提供了对北美输血前相容性检测实践和趋势的深入了解。
使用 CAP 能力验证数据评估当前北美用于 ABO 分组、Rh 定型、抗体筛查和交叉配血的手动检测实践。
对 CAP 实验室间比对计划 J 调查数据（2005-2010 年）进行分析，以确定用于 ABO 分组、Rh 定型、抗体筛查和交叉配血的实验室方法。使用 Microsoft（华盛顿州雷德蒙德）Excel 软件按测试方法对数据进行分析。
ABO 分组和 Rh 定型最常使用的方法是试管检测。许多实验室也使用试管检测进行抗体检测和交叉配血，但在研究期间，使用基于凝胶的方法的实验室比例显著增加。
大多数北美 CAP 实验室继续使用试管方法进行 ABO/Rh 检测。在过去的 5 年中，用于抗体筛查和交叉配血的基于凝胶的方法的使用有所增加。
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