Wilson Shanna M, Goldwasser Michael S, Clark Sherrie G, Monaco Elisa, Bionaz Massimo, Hurley Walter L, Rodriguez-Zas Sandra, Feng Liang, Dymon Zaneta, Wheeler Matthew B
Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
J Oral Maxillofac Surg. 2012 Mar;70(3):e193-203. doi: 10.1016/j.joms.2011.10.029.
This study investigated the effect of adipose-derived mesenchymal stem cells (ASCs) injected locally or systemically on the bone regeneration of a 10-mm-diameter cylindrical noncritical-size defect in the ramus of the pig mandible.
Fifteen Yorkshire pigs, weighing 60 to 80 kg, received bilateral 10-mm-diameter cylindrical surgical defects in each ramus of the mandible. Pigs received 1) a direct injection into the defect of 2.5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); 2) an ear vein injection of 5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); or 3) an ear vein injection of culture Dulbecco's Modified Eagle's Medium without stem cells (control; n = 3). Pigs from each treatment were sacrificed at 1 hour, 2 weeks, or 4 weeks after surgery. Healing of the defect was evaluated by dual-energy x-ray absorptiometry, micro-computed tomography, fluorescent microscopy, and histology.
Bone healing was accelerated in the ASC-injected treatment groups at 2 and 4 weeks after surgery compared with the control pigs.
Results from this animal model provide evidence that the injection of ASC locally into a bone defect or systemically can accelerate the healing of bone.
本研究调查了局部或全身注射脂肪来源间充质干细胞（ASC）对猪下颌支直径10mm的圆柱形非临界尺寸骨缺损骨再生的影响。
15只体重60至80kg的约克夏猪，每侧下颌支均接受直径10mm的圆柱形手术缺损。猪被分为三组：1）向缺损部位直接注射来自2头猪供体之一的250万个羧基荧光素二乙酸琥珀酰亚胺酯标记的ASC（n = 6）；2）经耳静脉注射来自2头猪供体之一的500万个羧基荧光素二乙酸琥珀酰亚胺酯标记的ASC（n = 6）；或3）经耳静脉注射不含干细胞的杜尔贝科改良伊格尔培养基（对照组；n = 3）。每组猪在术后1小时、2周或4周处死。通过双能X线吸收法、显微计算机断层扫描、荧光显微镜检查和组织学评估缺损的愈合情况。
与对照猪相比，术后2周和4周时，注射ASC的治疗组骨愈合加速。
该动物模型的结果提供了证据，表明将ASC局部注射到骨缺损中或全身注射可加速骨愈合。
