Zhang Lixia, Li Xuelan, Tang Deying, Guan Yanhong, Peng Jianming
Institute of Medicinal Plant Development Yunnan Branch, Chinese Academy of Medical Sciences, Jinghong 666100, China.
Zhongguo Zhong Yao Za Zhi. 2011 Nov;36(22):3086-90.
The aim of this study was to optimize the testing methods for seed quality, and to provide a basis for establishing seed testing criterion and quality standard of Amomum villosum.
Referring to the International Seed Testing Rules made by ISTA and Rules for agricultural seed testing, the seed quality of A. villosum from different collection areas was measured.
The samples weight of A. villosum for purity analysis were at least 500 g and for test were at least 50 g. Verification of genuineness was assayed by seed appearance comparing and weight of per hundred seeds was determined, the moisture content test was carried out by high temperature drying method (3 hours). The seeds were stored in wet sand for 20 days and then dipping in the 100 mg x L(-1) GA3 for 30 days before germination, seeds on filter papers germinated at 30/20 degrees C. The first germination-counting time was the 15th day of the test and the final time was the 50th day. Seed viability was tested by TTC method.
The seed testing methods for quality items of A. villosum, including sampling, purity analysis, verification of genuineness, weight, moisture content, percentage germination and seed viability of A. villosum had been initially established.
本研究旨在优化阳春砂仁种子质量检测方法，为制定阳春砂仁种子检测规程和质量标准提供依据。
参照国际种子检验协会（ISTA）制定的《国际种子检验规程》及《农作物种子检验规程》，对不同产地阳春砂仁种子质量进行测定。
阳春砂仁纯度分析试样重量至少500g，净度分析试样重量至少50g。通过种子外观比较进行真实性鉴定，测定千粒重，采用高温烘干法（3小时）进行水分测定。种子于湿沙中贮藏20天，发芽前于100mg·L⁻¹赤霉素溶液中浸种30天，于30/20℃在滤纸上发芽。初次计数时间为试验第15天，末次计数时间为第50天。采用TTC法测定种子生活力。
初步建立了阳春砂仁种子质量检测项目（包括取样、纯度分析、真实性鉴定、重量、水分、发芽率和种子生活力）的检测方法。
