Pfizer Analytical Research Centre, Australian Centre for Research On Separation Science, School of Chemistry, University of Tasmania, Private Bag 75, Hobart 7001, Australia.
J Chromatogr A. 2012 Apr 13;1233:71-7. doi: 10.1016/j.chroma.2012.02.008. Epub 2012 Feb 11.
For the hyphenation of ion chromatography to nebulising detectors or mass spectrometry, suppression of the non-volatile ionic eluent to water is a required step to avoid elevated detector baselines. Presented here is a study of three new designs of electrolytic suppressors, incorporating high ion-exchange capacity screens and high ion-exchange capacity membranes in different thickness and compositions. These designs aim to minimise hydrophobic interactions of the suppressor with organic analytes and to provide higher compatibility with eluents containing acetonitrile. In comparison with a commercially available electrolytic suppressor and also a commercially available chemical suppressor, the new high-capacity suppressor showed superior performance, exhibiting minimal interactions with a test set of analytes under the examined conditions. This led to the attainment of high recoveries of the analytes after suppression (93-99% recovery) and significantly reduced band broadening during suppression. The new suppressor has been shown to perform well under both isocratic and gradient elution conditions.
为了将离子色谱与雾化检测器或质谱联用，必须将不可挥发的离子洗脱液抑制到水相中，以避免检测器基线升高。本文研究了三种新型电解抑制器的设计，它们在不同的厚度和组成中采用了高离子交换容量筛网和高离子交换容量膜。这些设计旨在最小化抑制器与有机分析物的疏水相互作用，并提高与含乙腈的洗脱液的兼容性。与市售的电解抑制器和市售的化学抑制器相比，新型高容量抑制器表现出更好的性能，在检查条件下，对一组测试分析物的相互作用最小。这导致在抑制后（93-99%的回收率）获得了分析物的高回收率，并显著减少了抑制过程中的带宽展宽。新型抑制器在等度和梯度洗脱条件下均表现良好。
