Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA.
Analyst. 2012 Jul 7;137(13):2958-64. doi: 10.1039/c2an15868j. Epub 2012 Mar 1.
We have optimized an imaging methodology capable of monitoring individual live HeLa cells using non-synchrotron FTIR in an aqueous environment. This methodology, in combination with MATLAB based pre-processing techniques, allows fast and efficient collection of data with high signal-to-noise ratio in comparison with previous methods using point mode data collection, which required manual operation and more collection time. Also, presented are early results that illustrate interpretable spectral differences from live cells treated with chemotherapeutic drugs, demonstrating the potential of this methodology to develop more desirable modes of treatment for patients in their diagnoses and treatments for disease.
我们优化了一种成像方法，能够在水相环境中使用非同步傅里叶变换红外光谱（FTIR）监测单个活 HeLa 细胞。与之前使用点模式数据采集的方法相比，这种方法与基于 MATLAB 的预处理技术相结合，可以快速有效地收集具有高信噪比的数据，而之前的方法需要手动操作且收集时间更长。此外，本文还介绍了一些早期结果，这些结果表明，用化疗药物处理的活细胞在光谱上存在可解释的差异，这表明该方法有可能为患者的诊断和疾病治疗开发出更理想的治疗模式。
