Kemp B E, Pearson R B
St Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.
Trends Biochem Sci. 1990 Sep;15(9):342-6. doi: 10.1016/0968-0004(90)90073-k.
Protein kinases play a crucial role in the regulation of many cellular processes. They alter the functions of their target proteins by phosphorylating specific serine, threonine and tyrosine residues. Identification of phosphorylation site sequences and studies with corresponding model peptides have provided clues to how these important enzymes recognize their substrate proteins. This knowledge has made it possible to identify potential sites of phosphorylation in newly sequenced proteins as well as to construct specific model substrates and inhibitors.
蛋白激酶在许多细胞过程的调控中起着关键作用。它们通过磷酸化特定的丝氨酸、苏氨酸和酪氨酸残基来改变其靶蛋白的功能。磷酸化位点序列的鉴定以及对相应模型肽的研究为这些重要酶如何识别其底物蛋白提供了线索。这些知识使得识别新测序蛋白中潜在的磷酸化位点以及构建特定的模型底物和抑制剂成为可能。
