College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, People's Republic of China.
J Fish Biol. 2012 Mar;80(3):679-91. doi: 10.1111/j.1095-8649.2011.03203.x. Epub 2012 Feb 2.
Two complementary (c)DNA fragments, including the complete open reading frame of fabp2 from the common carp Cyprinus carpio, were cloned by reverse-transcription polymerase chain reaction (RT-PCR). Both were putative intestinal-type fabp genes, named fabp2a and fabp2b. fabp2b was mainly expressed in the intestine and the brain. This gene, however, was nearly not expressed in the liver, heart, pancreas and muscle. fabp2a was only expressed at a very low level in the intestine. Western blot also showed that Fabp2 is relatively highly expressed in the intestine and the brain. Immunohistochemical analysis revealed that Fabp2 is widely distributed in the mucosa of the intestine. These findings provide novel insights into the fabp2 gene molecular evolution, as well as its potential features in the intestine and the brain.
通过反转录聚合酶链反应（RT-PCR），我们从鲤鱼（Cyprinus carpio）克隆了两个互补的 cDNA 片段，其中包含完整的 fabp2 开放阅读框。这两个片段都是假定的肠道型 fabp 基因，分别命名为 fabp2a 和 fabp2b。fabp2b 主要在肠道和脑中表达。然而，该基因在肝脏、心脏、胰腺和肌肉中几乎不表达。fabp2a 仅在肠道中以极低的水平表达。Western blot 也表明 Fabp2 在肠道和脑中的表达相对较高。免疫组织化学分析显示 Fabp2 在肠道黏膜中广泛分布。这些发现为 fabp2 基因的分子进化及其在肠道和脑中的潜在特征提供了新的见解。
