Park Kyoung Sik, Kim Jong Bin, Lee Sun Joo, Bae Jaeman
Department of Surgery, Konkuk University School of Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
J Obstet Gynaecol Res. 2012 Mar;38(3):535-40. doi: 10.1111/j.1447-0756.2011.01743.x. Epub 2012 Feb 16.
The aim of this study is to analyze the impact of berberine on the two human epithelial ovarian carcinoma cell lines OVCAR-3 and SKOV-3 in relation to the potential usefulness of berberine in the treatment of epithelial ovarian cancer.
Under adherent culture conditions, the cell lines were treated with berberine and analyzed for changes in cell growth. The cell cycle duration and degree of apoptosis were evaluated by means of propidium iodide staining and Annexin V staining.
After the berberine treatment, the two cell lines showed a dose-dependent reduction in the growth rate. In the cell cycle analysis, the OVCAR-3 and SKOV-3 cells showed an increased DNA content of 5% in the G2/M phase and 7% in the S phase, respectively. Additionally, the results confirm the cell cycle arrest by immunoblotting and the up-regulation of p27; however, in the apoptosis analysis, neither cell line showed an increase in apoptosis after the berberine treatment.
Berberine treatment can inhibit proliferation through a cell cycle arrest in OVCAR-3 and SKOV-3 cells. Thus, berberine may be a novel anticancer drug for the treatment of ovarian cancer.
本研究旨在分析黄连素对两种人上皮性卵巢癌细胞系OVCAR - 3和SKOV - 3的影响，探讨黄连素在治疗上皮性卵巢癌方面的潜在效用。
在贴壁培养条件下，用黄连素处理细胞系，并分析细胞生长的变化。通过碘化丙啶染色和膜联蛋白V染色评估细胞周期持续时间和凋亡程度。
黄连素处理后，两种细胞系的生长速率均呈剂量依赖性降低。在细胞周期分析中，OVCAR - 3和SKOV - 3细胞在G2/M期的DNA含量分别增加了5%，在S期增加了7%。此外，结果通过免疫印迹证实了细胞周期停滞以及p27的上调；然而，在凋亡分析中，黄连素处理后两种细胞系均未显示凋亡增加。
黄连素处理可通过使OVCAR - 3和SKOV - 3细胞的细胞周期停滞来抑制增殖。因此，黄连素可能是一种治疗卵巢癌的新型抗癌药物。
