着丝粒蛋白H在人胃癌细胞增殖中的作用

[Role of centromere protein H in human gastric cancer cell proliferation].

作者信息

Quan Tianyi, He Benfu, Liu Tiejian, Li Weiming, Wu Shangbiao, Jiang Qingping, Liu Weiqiang, Liu Haibo, Xu Xuehu

机构信息

Department of Oncology, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2012 Feb;32(2):265-9.

PMID:22381774

Abstract

OBJECTIVE

To explore the role of centromere protein H (CENP-H) in the proliferation of human gastric cancer cells.

METHODS

RT-PCR and Western blot analysis were employed to examine the mRNA and protein expressions of CENP-H in 7 human gastric cancer cell lines and immortalized human gastric epithelial cells (GES-1). The cells were infected with the retrovirus vectors pMSCV-CENP-H or CENP-H-RNAi to establish stable cell lines with high CENP-H expression or CENP-H expression interference. MTT assay and colony formation assay were used to examine the changes in the cell proliferation after the infection.

RESULTS

CENP-H was over-expressed in gastric cancer cell lines AGS, BGC823, SGC-7901, MKN45, HGC27, MGC-803 and MKN28 at both mRNA and protein levels. The established AGS/CENP-H cell line with increased CENP-H expression showed enhanced proliferative activity, while the cell line MGC-803/CENP-H-RNAi with CENP-H expression interference showed an obviously lowered proliferation ability.

CONCLUSION

CENP-H promotes the proliferation of human gastric cancer cells, suggesting its important role in the occurrence and development of gastric cancer.

摘要

目的

探讨着丝粒蛋白H（CENP-H）在人胃癌细胞增殖中的作用。

方法

采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析检测7种人胃癌细胞系及永生化人胃上皮细胞（GES-1）中CENP-H的mRNA和蛋白表达。用逆转录病毒载体pMSCV-CENP-H或CENP-H-RNAi感染细胞，建立CENP-H高表达或CENP-H表达受干扰的稳定细胞系。采用MTT法和集落形成试验检测感染后细胞增殖的变化。

结果

CENP-H在胃癌细胞系AGS、BGC823、SGC-7901、MKN45、HGC27、MGC-803和MKN28中的mRNA和蛋白水平均呈过表达。建立的CENP-H表达增加的AGS/CENP-H细胞系增殖活性增强，而CENP-H表达受干扰的MGC-803/CENP-H-RNAi细胞系增殖能力明显降低。