在分泌性中耳炎患儿的肥大腺样体中，表达 CD127 和 CD132 的 T 细胞比例增加。

Increased percentage of T cells with the expression of CD127 and CD132 in hypertrophic adenoid in children with otitis media with effusion.

机构信息

Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Waszyngtona 17, 15-274 Bialystok, Poland.

出版信息

Eur Arch Otorhinolaryngol. 2012 Jul;269(7):1821-5. doi: 10.1007/s00405-012-1977-8. Epub 2012 Mar 2.

DOI:10.1007/s00405-012-1977-8

PMID:22382400

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3365238/

Abstract

The hypertrophic adenoid may promote chronic suppurative otitis media in children as it fulfills its immune function. The number of lymphocytes in the adenoid and their cooperation in the immune response depend of on their proliferation and migration to the effector sites. Interleukin 7 (IL-7) is essential for the normal development and function lymphocytes. IL-7 plays pivotal role for activation and proliferation of T and B cells. The heterodimeric interleukin-7 receptor (IL-7R) is composed of the IL-7Rα (127) and the common cytokine receptor γc (CD132). The aim of this study was to evaluate the percentage of lymphocytes T (CD4(+) and CD8(+)) with IL-7R (CD127 and CD132) expression in hypertrophic adenoid in children suffering with otitis media with effusion for a duration of 3 months. Adenoid excised due to hypertrophy with or without chronic otitis media with effusion was used as study material. CD4(+) CD127(+), CD4(+)132(+), CD8(+)CD127(+) and CD8(+)CD132(+) cell subpopulations were identified using monoclonal antibodies and flow cytometry. The percentage of CD4(+) and CD8(+) T cells with CD127 receptor expression in hypertrophic adenoid of children with otitis media with effusion was statistically significantly higher than in hypertrophic adenoid group. The percentage of CD4(+) T cells with CD132 expression in the study group was statistically significantly higher than in the reference group. The percentage of CD8(+) T cells with CD132(+) expression was not statistically different in both groups. The increased percentage of T lymphocytes with IL-7R expression (CD127 and CD132) in hypertrophic adenoid seems to influence the quantity of lymphocytes and upset the immunological function of tonsils which can influence the course of otitis media with effusion.

摘要

肥大的腺样体可能会促进儿童慢性化脓性中耳炎，因为它完成了免疫功能。腺样体中淋巴细胞的数量及其在免疫反应中的协同作用取决于它们的增殖和向效应部位的迁移。白细胞介素 7（IL-7）是淋巴细胞正常发育和功能所必需的。IL-7 在 T 和 B 细胞的激活和增殖中起着关键作用。白细胞介素 7 受体（IL-7R）是由 IL-7Rα（127）和共同细胞因子受体γc（CD132）组成的异二聚体。本研究旨在评估患有分泌性中耳炎的儿童肥大腺样体中表达 IL-7R（CD127 和 CD132）的 T 淋巴细胞（CD4(+)和 CD8(+)）的百分比，持续时间为 3 个月。肥大的腺样体因肥大伴或不伴慢性分泌性中耳炎而被切除作为研究材料。使用单克隆抗体和流式细胞术鉴定 CD4(+) CD127(+)、CD4(+)132(+)、CD8(+)CD127(+)和 CD8(+)CD132(+)细胞亚群。患有分泌性中耳炎的儿童肥大腺样体中表达 CD127 受体的 CD4(+)和 CD8(+)T 细胞的百分比明显高于肥大腺样体组。研究组中 CD4(+) T 细胞表达 CD132 的百分比明显高于对照组。两组中 CD8(+) T 细胞表达 CD132(+)的百分比无统计学差异。肥大腺样体中表达 IL-7R（CD127 和 CD132）的 T 淋巴细胞的百分比增加似乎影响了淋巴细胞的数量，并扰乱了扁桃体的免疫功能，从而影响了分泌性中耳炎的病程。