[分泌性中耳炎患儿腺样体肥大中T淋巴细胞和B淋巴细胞Bcl-2蛋白的表达]
[Expression Bcl-2 protein of lymphocytes T and B in hypertrophied adenoid in children with otitis media with effusion].
作者信息
Zelazowska-Rutkowska Beata, Wysocka Jolanta, Hassmann-Poznańska Elzbieta, Zak Janusz
机构信息
Zakład Laboratoryjnej Diagnostyki Pediatrycznej AM w Białymstoku.
出版信息
Otolaryngol Pol. 2006;60(5):737-42.
INTRODUCTION
The quantity of lymphocytes in adenoid and their cooperation in immunological processes is dependent on proliferation process and their migration to the effectory places. The most important process provide for equilibrium between lymphocytes of tonsils is apopotosis. Special meaning in induction this process has Bcl-2 family proteins with can be divided into pro-apoptotic and anti-apoptotic. An investigation was executed in hypertrophied adenoids with or without otitis media with effusion.
MATERIAL AND METHODS
By flow cytometry percentage of lymphocytes CD4+, CD8+, CD19+ and percentage of these lymphocytes with expression Bcl-2 proteins in hypertrophied adenoid and hypertrophied adenoid and otitis media with effusion was analyzed.
RESULTS
The percentage of lymphocytes CD4+Bcl-2+, CD8+Bcl-2+, CD19+Bcl-2+ was lower in hypertrophied adenoid and otitis media with effusion compared to the control group.
CONCLUSIONS
Tendency to lowered percentage of lymphocytes T and B with expression Bcl -2 protein in adenoids reflects likely the local immunity alterations.
引言
腺样体中淋巴细胞的数量及其在免疫过程中的协同作用取决于增殖过程以及它们向效应部位的迁移。扁桃体淋巴细胞之间实现平衡的最重要过程是细胞凋亡。在诱导这一过程中，具有促凋亡和抗凋亡之分的Bcl-2家族蛋白具有特殊意义。对伴有或不伴有分泌性中耳炎的腺样体肥大患者进行了一项研究。
材料与方法
通过流式细胞术分析腺样体肥大患者以及腺样体肥大合并分泌性中耳炎患者中CD4+、CD8+、CD19+淋巴细胞的百分比以及这些淋巴细胞中表达Bcl-2蛋白的百分比。
结果
与对照组相比，腺样体肥大合并分泌性中耳炎患者中CD4+Bcl-2+、CD8+Bcl-2+、CD19+Bcl-2+淋巴细胞的百分比更低。
结论
腺样体中表达Bcl-2蛋白的T淋巴细胞和B淋巴细胞百分比有降低的趋势，这可能反映了局部免疫改变。
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