Fathy F A, Bursian S
Department of Animal Science, Michigan State University, East Lansing 48824.
Vet Hum Toxicol. 1990 Oct;32(5):416-8.
Immature cockerels were susceptible to OPIDN when dosed with TOCP. Using 30 broiler-breed cockerels, 6 w old, 10 birds each received 28 daily im injections of either 50 mg estradiol, 100 mg testosterone or 0.1 ml of vehicle. At 7 w of age, 5 birds in each of the 3 groups received a single oral dose of 500 mg TOCP/kg body weight, while the remaining 5 birds/groups were given corn oil. The birds were observed daily for 14 d beginning on day 8 post-TOCP exposure for the development of clinical signs characteristic of OPIDN. At 21 d post-TOCP, all birds were killed and the adrenal gland and testes were prepared for histopathology of the birds that received TOCP, 1 of 5 that were given testosterone and 2 of 5 that received estradiol had signs typical of OPIDN. All of the 5 birds that received TOCP alone showed OPIDN signs. The testes of the TOCP-exposed birds that showed clinical signs had reductions in the size of the seminiferous tubules and no evidence of spermatogenic activity. This study demonstrated that sex hormones can modulate the clinical effects of TOCP in immature cockerels through unknown mechanisms that are similar to those reported for corticosterone in adult chickens.
未成熟的小公鸡经三邻甲苯磷酸酯（TOCP）给药后易患迟发性神经病变（OPIDN）。选用30只6周龄的肉用种小公鸡，每组10只，分别每日肌肉注射28次，每次剂量为50毫克雌二醇、100毫克睾酮或0.1毫升赋形剂。7周龄时，3组中的每组5只鸡口服单剂量500毫克TOCP/千克体重，其余每组5只鸡给予玉米油。从TOCP暴露后第8天开始，每天观察鸡14天，观察OPIDN特征性临床症状的发展情况。TOCP给药后21天，所有鸡均处死后，对接受TOCP的鸡的肾上腺和睾丸进行组织病理学检查，接受睾酮的5只中有1只、接受雌二醇的5只中有2只出现OPIDN典型症状。仅接受TOCP的5只鸡均出现OPIDN症状。出现临床症状的TOCP暴露鸡的睾丸生精小管大小减小，且无生精活性迹象。本研究表明，性激素可通过未知机制调节TOCP对未成熟小公鸡的临床作用，这些机制与成年鸡中报道的皮质酮的机制相似。
