Sun Xing-hua, Zhang Li-qiang, He Bei
Department of Respiratory Medicine, Peking University Third Hospital, Beijing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Dec;31(12):1680-4.
To study the effects of Compound Danshen Dripping Pill (CDDP) on the structure and functions of sternohyoid muscle in metabolic syndrome (MS) rats, and to study whether it has therapeutic effects on obstructive sleep apnea-hypopnea syndrome (OSAHS).
Twenty-one healthy male SD rats were randomly divided into three groups, i.e., the normal control group (n = 6), the MS group (n = 8), and the CDDP group (n = 7). Rats in the normal control group were routinely fed. High lipid forage was given to rats in the rest two groups. Nine weeks later, CDDP (at the dose of 375 mg/kg) was additionally given to rats in the CDDP group by gastrogavage, and then rats in the CDDP group and the MS group were fed with the same high lipid forage for 12 successive weeks. The content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) in the sternohyoid muscle were detected in the three groups. The capillary density, capillary-to-fiber ratio (C/F), the section area of type I muscle fiber were detected using myosin-ATPase histochemical assay. The contractile changes of isometric stemohyoid muscles were determined under electric stimulation by different frequencies.
The contents of MDA were obviously lower in the CDDP group than in the MS group, while the activities of SOD, the capillary density, C/F, the section area of type I muscle fiber, the tension of stemohyoid muscle at 10 -60 Hz, and the 1-5 min tension percentages of the stemohyoid muscle were higher in the CDDP group than in the MS group (P < 0.05, P < 0.01).
CDDP could improve oxidative stress induced intramuscularly microcirculation disturbance and changes of muscular fiber structures of the upper airway muscles, and elevate their contractile functions, thus possibly contributing to favorable effects on OSAHS.
研究复方丹参滴丸（CDDP）对代谢综合征（MS）大鼠胸骨舌骨肌结构和功能的影响，探讨其对阻塞性睡眠呼吸暂停低通气综合征（OSAHS）是否具有治疗作用。
将21只健康雄性SD大鼠随机分为三组，即正常对照组（n = 6）、MS组（n = 8）和CDDP组（n = 7）。正常对照组大鼠常规饲养。其余两组大鼠给予高脂饲料。9周后，CDDP组大鼠额外灌胃给予CDDP（剂量为375 mg/kg），然后CDDP组和MS组大鼠继续喂饲相同高脂饲料12周。检测三组大鼠胸骨舌骨肌中丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。采用肌球蛋白 - ATP酶组织化学法检测毛细血管密度、毛细血管与肌纤维比值（C/F）、I型肌纤维截面积。在不同频率电刺激下测定等长胸骨舌骨肌的收缩变化。
CDDP组MDA含量明显低于MS组，而CDDP组SOD活性、毛细血管密度、C/F、I型肌纤维截面积、10 - 60 Hz时胸骨舌骨肌张力以及胸骨舌骨肌1 - 5分钟张力百分比均高于MS组（P < 0.05，P < 0.01）。
CDDP可改善氧化应激诱导的上气道肌肉内微循环障碍和肌纤维结构改变，提高其收缩功能，从而可能对OSAHS产生有益作用。
