Liu Ying-ke, Zhang Zhi-xin, Zhang Qiong
Department of Pediatrics, China-Japan Friendship Hospital, Beijing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Dec;31(12):1690-4.
To study the effect of growth hormone (GH) combined with Radix Dipsaci on the body growth and the bone mineral content (BMC) of hypophysectomized rats.
The GH deficiency rats model was established using the hypophysectomized operation through the skull and the throat. Qualified rats were divided into the sham-operation group (n = 15), the negative control group (n = 13), the GH intervention group (n = 13), and the GH combined with Radix Dipsaci group(n = 12). GH (0.25 mg/kg) was subcutaneously injected from the cervical part in the GH intervention group and the GH combined with Radix Dipsaci group at the same time, while equal volume of normal saline was injected to the rest groups. 0.7 mL/100 kg Radix Dipsaci was given by gastrogavage to the GH combined with Radix Dipsaci group at the same time, while equal volume of normal saline was given by gastrogave to the rest groups. The body weight, the tail length, and the body length were measured during the intervention period. Blood was withdrawn after 14-day intervention. The femoral bone and the tibial bone were taken out. The levels of GH, insulin-like growth factor 1 (IGF-1), alkaline phosphatase (ALP), and osteocalcin (OC) were measured. The width of the tibial epiphyseal plate was measured. The bilateral femur bone mineral density (BMD) and BMC were measured using the dual energy X-ray absorptiometry.
The body weight, the body length, the length of the femoral bone, the length of the tibial bone, the width of the epiphyseal plate, the levels of the GH, IGF-1, ALP, and OC increased in the GH intervention group and the GH combined with Radix Dipsaci group after 2-week intervention, showing statistical difference when compared with the model group (P < 0.01). But there was no statistical difference in the tail length though it also increased (P > 0.05). There was insignificant difference in the aforesaid indices between the two groups (P > 0.05). Compared with the model group, the BMD of the GH combined with Radix Dipsaci group increased with statistical difference (P < 0.01). Compared with the model group, the BMC of the GH intervention group and the GH combined with Radix Dipsaci group increased with statistical difference (P < 0.01). It was highest in the GH combined with Radix Dipsaci group (P < 0.01).
GH combined with Radix Dipsaci showed unobvious effect on promoting the growth. But it could elevate BMD and BMC, and improve the bone metabolism.
研究生长激素（GH）联合续断对垂体切除大鼠身体生长及骨矿物质含量（BMC）的影响。
采用经颅和经喉垂体切除手术建立GH缺乏大鼠模型。将合格大鼠分为假手术组（n = 15）、阴性对照组（n = 13）、GH干预组（n = 13）和GH联合续断组（n = 12）。GH干预组和GH联合续断组自颈部皮下注射GH（0.25 mg/kg），其余组注射等体积生理盐水。GH联合续断组同时灌胃给予0.7 mL/100 kg续断，其余组灌胃等体积生理盐水。干预期间测量体重、尾长及体长。干预14 d后采血，取出股骨和胫骨，检测GH、胰岛素样生长因子1（IGF-1）、碱性磷酸酶（ALP）及骨钙素（OC）水平，测量胫骨骨骺板宽度，采用双能X线吸收法测量双侧股骨骨密度（BMD）和BMC。
干预2周后，GH干预组和GH联合续断组的体重、体长、股骨长度、胫骨长度、骨骺板宽度、GH、IGF-1、ALP及OC水平均升高，与模型组比较差异有统计学意义（P < 0.01），尾长虽也增加但差异无统计学意义（P > 0.05）。两组上述指标比较差异无统计学意义（P > 0.05）。与模型组比较，GH联合续断组BMD升高差异有统计学意义（P < 0.01）。与模型组比较，GH干预组和GH联合续断组BMC升高差异有统计学意义（P < 0.01），其中GH联合续断组最高（P < 0.01）。
GH联合续断促进生长作用不明显，但可提高BMD和BMC，改善骨代谢。
