Yue Bing-Bing, Li Xin, Ren Fang-Fei, Meng Fan-Juan, Yin Peng-Da, Zhang Hui-Hui, Sun Guang-Yu
College of Life Science, Northeast Forestry University, Harbin 150040, China.
Ying Yong Sheng Tai Xue Bao. 2011 Dec;22(12):3259-64.
By using Biolog technique, this paper studied the carbon source utilization characteristics of microbial communities in different layers (0-10 cm, 10-20 cm, and 20-30 cm) of a petroleum-contaminated soil near an oil well having exploited for 36 years in Daqing Oil Field. Petroleum contamination enhanced the metabolic activity of the soil microbial communities obviously. In the three layers of the petroleum-contaminated soil, the metabolic activity of the microbes was higher than that of the control, and there existed significant differences between different layers of the petroleum-contaminated soil. The carbon source metabolic capacity of the microbes in different layers of the petroleum-contaminated soil was in the order of 20-30 cm > 10-20 cm > 0-10 cm. Petroleum contamination made the kinds of soil carbon source and the metabolic diversity of soil microbes increased, being more obvious in 10-20 cm and 20-30 cm soil layers but less change in 0-10 cm soil layer. In the contaminated soil, the majority of the carbon sources utilized by the microbes in 10-20 cm soil layer were carbohydrates instead of the carboxylic acids in non-contaminated soil, whereas the majority of the carbon substrates utilized in 20-30 cm soil layer were carboxylic acids. All the results suggested that petroleum-contaminated soil had its unique microbial community structure and peculiar microbial carbon source utilization characteristics.
利用Biolog技术，研究了大庆油田某开采36年的油井附近石油污染土壤不同层次（0 - 10厘米、10 - 20厘米和20 - 30厘米）微生物群落的碳源利用特征。石油污染明显增强了土壤微生物群落的代谢活性。在石油污染土壤的三个层次中，微生物的代谢活性均高于对照，且石油污染土壤不同层次间存在显著差异。石油污染土壤不同层次微生物的碳源代谢能力顺序为20 - 30厘米＞10 - 20厘米＞0 - 10厘米。石油污染使土壤碳源种类和土壤微生物代谢多样性增加，在10 - 20厘米和20 - 30厘米土层更为明显，而在0 - 10厘米土层变化较小。在污染土壤中，10 - 20厘米土层微生物利用的碳源多数为碳水化合物，而非未污染土壤中的羧酸类物质，而20 - 30厘米土层利用的碳源多数为羧酸类物质。所有结果表明，石油污染土壤具有独特的微生物群落结构和特殊的微生物碳源利用特征。
