Dobrowolski Dariusz, Wylegala Edward, Orzechowska-Wylegala Bogusława, Wowra Bogumił, Wróblewska-Czajka Ewa
Department of Ophthalmology, District Railway Hospital, Katowice, Poland.
Klin Oczna. 2011;113(10-12):346-51.
To present results of ocular surface reconstruction with application of cultured corneal epithelium in limbal stem cell insufficiency.
25 patients suffered from limbal insufficiency in one eye after a chemical or thermal burn. Their healthy eyes were the source of limbal epithelium for corneal epithelial culture. Limbal cells from a 2 mm2 biopsy were seeded on amniotic membrane. Cultures were carried out in standard conditions in supplemented DMEM in presence of 3T3 fibroblasts. After superficial keratectomy the amniotic carriers with epithelial cells were transplanted on denuded corneas. The presence of corneal vascularization, epithelial regularity and visual acuity were evaluated.
Postoperative follow-up ranged from 3 up to 9 months, average 4.9 +/- 1.1 months. 3 months after the surgery 72.0% of the eyes showed stable epithelium with slight corneal haze caused by the amnion. In 60.0% of the eyes there was no recurrent conjunctival neovascularization. 28.0% of the eyes remained cloudy due to stromal revascularization. In 2 eyes total conjunctival pannus developed again. Visual acuity ranged from counting fingers to 0.6.
Transplantation of cultured epithelium is a promising method in treatment of limbal stem cell insufficiency giving satisfactory restoration of corneal epithelium.
介绍应用培养的角膜上皮进行眼表重建治疗角膜缘干细胞功能不全的结果。
25例患者因化学或热烧伤导致单眼角膜缘功能不全。其健眼作为角膜上皮培养的角膜缘上皮来源。将取自2mm²活检组织的角膜缘细胞接种于羊膜上。在添加了3T3成纤维细胞的补充DMEM培养基中，于标准条件下进行培养。在浅表角膜切除术后，将带有上皮细胞的羊膜载体移植到裸露的角膜上。评估角膜血管化、上皮规则性和视力情况。
术后随访时间为3至9个月，平均4.9±1.1个月。术后3个月，72.0%的眼表上皮稳定，伴有羊膜引起的轻微角膜混浊。60.0%的眼无结膜新生血管复发。28.0%的眼因基质血管化而仍混浊。2只眼再次出现完全性结膜血管翳。视力范围从指数到0.6。
培养上皮移植是治疗角膜缘干细胞功能不全的一种有前景的方法，可使角膜上皮得到满意的恢复。
