Trzcińiska Agnieszka, Laskowska Anna, Siennicka Joanna
Zakład Wirusologii NIZP-PZH w Warszawie.
Med Dosw Mikrobiol. 2011;63(4):341-7.
Obtaining a reliable laboratory test result depends on many factors, among which preanalitical factors play a significant role. The aim of this study was to determine the effect of temperature, storage time of samples and number of freezing and thawing cycles on the results of tests carried out by RT-PCR. The study was conducted in a model of measles virus (RNA). The results revealed that: (1) Samples of clinical material (serum, cerebrospinal fluid, urine) for testing by RT-PCR can be stored for at least 5 days at room temperature or refrigerated and at least 7 days when frozen, (2) Freezing and thawing three times, samples of clinical material does not affect the outcome of the presence of viral RNA, (3) Isolated viral RNA is stable at freezer temperature and can be subjected to at least 20 freeze-thaw cycles without affecting the results.
获得可靠的实验室检测结果取决于许多因素，其中分析前因素起着重要作用。本研究的目的是确定温度、样本储存时间以及冻融循环次数对逆转录聚合酶链反应（RT-PCR）检测结果的影响。该研究以麻疹病毒（RNA）为模型进行。结果显示：（1）用于RT-PCR检测的临床材料（血清、脑脊液、尿液）样本在室温或冷藏条件下可保存至少5天，冷冻条件下可保存至少7天；（2）临床材料样本冻融三次不影响病毒RNA存在的检测结果；（3）分离出的病毒RNA在冷冻温度下稳定，可经受至少20次冻融循环而不影响结果。
