在表达病毒外壳蛋白基因的转基因烟草植物原生质体中对烟草花叶病毒颗粒脱壳的抑制作用。

Inhibition of uncoating of tobacco mosaic virus particles in protoplasts from transgenic tobacco plants that express the viral coat protein gene.

作者信息

Wu X J, Beachy R N, Wilson T M, Shaw J G

机构信息

Department of Plant Pathology, University of Kentucky, Lexington 40546.

出版信息

Virology. 1990 Dec;179(2):893-5. doi: 10.1016/0042-6822(90)90163-l.

DOI:10.1016/0042-6822(90)90163-l

PMID:2238480

Abstract

The uncoating of tobacco mosaic virus (TMV) particles in protoplasts isolated from leaves of transgenic tobacco plants that express the TMV coat protein gene was investigated. Extracts of these protoplasts collected up to 1 hr after inoculation with TMV contained fewer of the complexes ("striposomes") thought to be involved in cotranslational disassembly of virus particles than did extracts of protoplasts that do not express the viral coat protein gene. These results are consistent with the hypothesis that TMV coat protein-mediated resistance is at least in part the result of inhibition of the uncoating of the virus particles in the inoculum.

摘要

对从表达烟草花叶病毒（TMV）外壳蛋白基因的转基因烟草植株叶片中分离出的原生质体中TMV颗粒的脱壳情况进行了研究。接种TMV后1小时内收集的这些原生质体提取物中，被认为参与病毒颗粒共翻译拆解的复合物（“条状体”）比未表达病毒外壳蛋白基因的原生质体提取物中的要少。这些结果与以下假设一致，即TMV外壳蛋白介导的抗性至少部分是接种物中病毒颗粒脱壳受到抑制的结果。